Abstract：
White Leghorn chickens from a common founder population have been divergently selected for high (HAS) or low (LAS) antibody responses to sheep red blood cells (SRBC) for 49 generations resulting in 2 diverse lines for this trait. Much has been studied in these two lines; however, the impact of these selection pressures on cytokine and chemokine expression is not fully understood. The purpose of this study is to determine if selection for antibody response to SRBC impacts cytokine and chemokine expression in peripheral blood leukocytes (PBL) and spleen from HAS and LAS chickens. Total RNA was isolated from PBL and spleen after which mRNA expression of cytokines (IL4, IL6, IL10, TGF-β4) and chemokines (CXCL8, CCL4) were determined by quantitative real-time RT-PCR (qRT-PCR). The data were analyzed using Student\'s t test comparing HAS and LAS (P < 0.05) and are reported as corrected 40-CT. PBL and spleen samples were analyzed separately. With respect to PBL, expression of IL6 was higher (P < 0.05) in PBL isolated from LAS chickens compared to those from the HAS line whereas there were no differences (P > 0.05) in IL4, IL10, CXCL8, CCL4, or TGF-β4. The cytokine and chemokine mRNA expression profiles were different in the spleen between the two lines. IL4 and CXCL8 expression were higher (P < 0.05) in spleen samples from HAS chickens than LAS. The expression of IL6, IL10, CCL4, or TGF-β4 in the spleens did not differ (P > 0.05) between the lines. The data indicate that selection for specific antibody responses to SRBC impacts the cytokine and chemokine expression profile in PBL and spleens but in different ways in HAS and LAS. These studies provide insight into the influence that selection pressures for antibody responses have on different immune response components, specifically cytokines and chemokines typically involved in the innate response.
摘要：
来自普通创始人群体的白来汉鸡被不同地选择了49代对绵羊红细胞（SRBC）的高（HAS）或低（LAS）抗体反应，从而产生了2种不同的品系。在这两条线上已经研究了很多；然而，这些选择压力对细胞因子和趋化因子表达的影响尚不完全清楚.这项研究的目的是确定对SRBC的抗体应答的选择是否影响HAS和LAS鸡的外周血白细胞（PBL）和脾脏中的细胞因子和趋化因子表达。从PBL和脾分离总RNA,之后通过定量实时RT-PCR(qRT-PCR)测定细胞因子(IL4、IL6、IL10、TGF-β4)和趋化因子(CXCL8、CCL4)的mRNA表达。使用Studentt检验分析数据,比较HAS和LAS(P<0.05),并报告为校正的40-CT。分别分析PBL和脾样品。关于PBL，与HAS系相比，从LAS鸡分离的PBL中IL6的表达更高（P<0.05），而在IL4，IL10，CXCL8，CCL4或TGF-β4中没有差异（P>0.05）。两种细胞系之间的脾脏中细胞因子和趋化因子mRNA表达谱不同。HAS鸡的脾脏样本中IL4和CXCL8的表达高于LAS（P<0.05）。脾脏中IL6，IL10，CCL4或TGF-β4的表达在品系之间没有差异（P>0.05）。数据表明,针对SRBC的特异性抗体应答的选择影响PBL和脾脏中的细胞因子和趋化因子表达谱,但在HAS和LAS中以不同的方式。这些研究提供了对抗体反应的选择压力对不同免疫反应成分的影响的见解。特别是细胞因子和趋化因子通常参与先天反应。
