Abstract：
Representatives of the genus Acanthamoeba are among the most widespread protists in the environment. They have a ubiquitous distribution and can sometimes cause quite serious pathologies in humans. The treatment ofp rotozoal infections caused by free-living amoebae is currently limited and often unsuccessful. In the presented investigation, amebicidal activity was determined against both the trophozoites and cysts of Acanthamoeba spp., which were isolated during the microbiological examination of environmental objects. The inhibitory activity of drugs in vitro was determined using the authors\' proposed method, which is based on the plaque formation phenomenon: this is initiated by free-living amoebae when cultured in agar containing the bacteria Cellulosimicrobium sp. strain bent-1. Based on a series of experimental studies, the paper proposes a reliable and inexpensive method for determining the anti-protozoal activity of medicinal agents, which will significantly complement the current screening method system when studying existing drugs, or new drugs during their development stage.
摘要：
棘阿米巴属的代表是环境中最广泛的原生生物之一。它们具有普遍存在的分布，有时会在人类中引起相当严重的病变。目前，由自由生活的变形虫引起的轮虫感染的治疗有限，而且通常不成功。在提出的调查中，对棘阿米巴的滋养体和囊肿均测定了杀菌活性。,在环境对象的微生物检查过程中被分离。使用作者提出的方法测定药物的体外抑制活性，这是基于菌斑形成现象：这是由在含有细菌Cellulosimicrobiumsp。的琼脂中培养时的自由生活变形虫引发的。应变弯曲-1。基于一系列的实验研究,本文提出了一种可靠且廉价的方法来确定药物的抗原生动物活性，这将在研究现有药物时显著补充目前的筛选方法系统，或在其发展阶段的新药。
