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Abstract：
Colorectal cancer (CRC) is the third most common malignancy cause of cancer-related mortality worldwide. Epithelial-mesenchymal transition (EMT) promotes cancer metastasis and a tumour-based Glasgow EMT score was associated with adverse clinical features and poor prognosis. In this study, the impact of using the established five tumour-based EMT markers consisting of E-cadherin (E-cad), β-catenin (β-cat), Snail, Zeb-1, and Fascin in combination with the stromal periostin (PN) on the prediction of CRC patients\' prognosis were invesigated. Formalin-fixed paraffin-embedded tissues of 202 CRC patients were studies the expressions of E-cad, β-cat, Snail, Zeb-1, Fascin, and PN by immunohistochemistry. Individually, cytoplasmic Fascin (Fc), cytoplasmic Snail (Sc), nuclear Snail (Sn), stromal Snail (Ss), and stromal PN (Ps) were significantly associated with reduced survival. A combination of Ps with Fc, Fs, and Sn was observed in 2 patterns including combined Fc, Fs, and Ps (FcFsPs) and Fc, Sn, and Ps (FcSnPs). These combinations enhanced the prognostic power compared to individual EMT markers and were independent prognostic markers. As the previously established scoring method required five markers and stringent criteria, its clinical use might be limited. Therefore, using these novel combined prognostic markers, either FcFsPs or FcSnPs, may be useful in predicting CRC patient outcomes.
摘要：
结直肠癌（CRC）是全球癌症相关死亡率的第三大常见恶性肿瘤。上皮-间质转化（EMT）促进癌症转移，基于肿瘤的格拉斯哥EMT评分与不良临床特征和不良预后相关。在这项研究中,使用由E-cadherin（E-cad）组成的五种基于肿瘤的EMT标志物的影响，β-连环蛋白(β-cat)，蜗牛,研究了Zeb-1和Fascin联合基质骨膜素（PN）对预测CRC患者预后的影响。研究了202例CRC患者福尔马林固定石蜡包埋组织中E-cad的表达，β-cat,蜗牛,Zeb-1,Fascin,和PN通过免疫组织化学。个别地,细胞质血管素（Fc），细胞质蜗牛（Sc），核蜗牛（Sn），基质蜗牛（Ss），和基质PN（Ps）与生存率降低显着相关。Ps与Fc的组合，Fs,和Sn在2种模式中观察到，包括组合Fc，Fs,和Ps（FcFsPs）和Fc，Sn,和Ps(FcSnPs)。与个体EMT标志物相比,这些组合增强了预后能力,并且是独立的预后标志物。由于先前建立的评分方法需要五个标记和严格的标准，其临床应用可能有限。因此,使用这些新的联合预后标志物，FcFsPs或FcSnPs，可能有助于预测CRC患者的预后。
