Abstract：
The biobanks from dermal biopsies represent an interesting strategy for biodiversity conservation. Nevertheless, the morphological and cellular patterns of the dermis can be influenced by the age and sex of the individual. Therefore, evaluating these factors is interesting for forming biobanks of Antillean manatees. These animals, representatives of marine fauna, have had their population reduced, and biobanks are essential for their conservation. Then, we evaluated the effects of age (3.5 years vs. 3.6-16 years vs. 23.6 years) and sex (males vs. females) on morphological and cellular parameters using histological and in vitro culture techniques. Regardless of age, no differences were observed for dermal thickness, collagen fibres, tissue proliferative activity and viable cell recovery. Nonetheless, fibroblast reduction was observed in groups aged 23.6 years compared to other animals (p < 0.05). Additionally, cells from animals aged 3.6-16 years showed more significant mitochondrial damage than the other groups (p < 0.05). Regardless of sex, no differences were observed for dermal thickness, collagen fibres, tissue proliferative activity and viable cell recovery; however, females had fewer fibroblasts than males (p < 0.05). Cells from females showed lower mitochondrial damage when compared to cells from males. In summary, although age and sex do not influence dermal thickness and cell recovery, variations in the number of fibroblasts and mitochondrial characteristics were observed among the groups. These differences may be significant for understanding the dermis aspects to be correlated to biobank systems.
摘要：
来自皮肤活检的生物库代表了生物多样性保护的有趣策略。然而，真皮的形态和细胞模式可受个体的年龄和性别的影响。因此,评估这些因素对于形成安的列尔海牛的生物库很有趣。这些动物,海洋动物的代表，他们的人口减少了，生物银行对它们的保护至关重要。然后，我们评估了年龄的影响(3.5岁与3.6-16年vs.23.6岁）和性别（男性vs.雌性）使用组织学和体外培养技术对形态和细胞参数进行研究。不管年龄,真皮厚度没有观察到差异，胶原纤维,组织增殖活性和活细胞恢复。尽管如此，与其他动物相比，在23.6岁的组中观察到成纤维细胞减少（p<0.05）。此外,来自3.6-16岁动物细胞的线粒体损伤比其他组更明显(p<0.05)。不管性别,真皮厚度没有观察到差异，胶原纤维,组织增殖活性和活细胞恢复;然而，女性的成纤维细胞少于男性（p<0.05）。与雄性细胞相比，雌性细胞的线粒体损伤较低。总之,虽然年龄和性别不影响皮肤厚度和细胞恢复，各组间观察到成纤维细胞数量和线粒体特征的变化.这些差异对于理解与生物库系统相关的真皮方面可能是重要的。
