Abstract：
Reniform and root-knot nematode are two of the most destructive pests of conventional upland cotton, Gossypium hirsutum, L. and continue to be a major threat to cotton fiber production in semi-arid regions of the southern United States and Central America. Fortunately, naturally occurring tolerance to these nematodes has been identified in the Pima cotton species (G. barbadense) and several upland cotton varieties (G. hirsutum), which has led to a robust breeding program that has successfully introgressed and stacked these independent resistant traits into several upland cotton lineages with superior agronomic traits, e.g. BAR 32-30 and BARBREN-713. This work identifies the genomic variations of these nematode tolerant accessions by comparing their respective genomes to the susceptible, high-quality fiber producing parental line of this lineage: Phytogen 355 (PSC355). We discover several large genomic differences within marker regions that harbor putative resistance genes as well as expression mechanisms shared by the two resistant lines, with respect to the susceptible PSC355 parental line. This work emphasizes the utility of whole genome comparisons as a means of elucidating large and small nuclear differences by lineage and phenotype. .
摘要：
肾形线虫和根结线虫是常规陆地棉最具破坏性的两种害虫,陆地棉,并继续对美国南部和中美洲半干旱地区的棉纤维生产构成重大威胁。幸运的是,在皮马棉种中已鉴定出对这些线虫的天然耐受性（G.巴巴多斯)和几个陆地棉品种(G.hirsutum),这导致了一个强大的育种计划，该计划已成功地将这些独立的抗性性状渗入并堆叠成具有优越农艺性状的几个陆地棉谱系，例如BAR32-30和BARBREN-713。这项工作通过将它们各自的基因组与易感基因组进行比较来识别这些线虫耐受性种质的基因组变异，该谱系的高质量纤维生产亲本系：Phytogen355（PSC355）。我们发现标记区域内存在几个巨大的基因组差异，这些差异包含推定的抗性基因以及两个抗性品系共有的表达机制。关于易感PSC355亲本系。这项工作强调了全基因组比较的实用性，可以通过谱系和表型阐明大小核差异。.
