Abstract：
Gut microbiota of the bumblebee is critical as it modulates the health and fitness of the host. However, the mechanisms underlying the formation and maintenance of the diversity of bumblebee gut bacteria over a long period of evolution have yet to be elucidated. In particular, the gut bacterial diversity and community assembly processes of Bombus pyrosoma across the Chinese border remain unclear. In this study, we systematically carried out unprecedented sampling of 513 workers of the species Bombus pyrosoma across the Chinese landscape and used full-length 16S rRNA gene sequencing to examine their gut microbiota diversity and biogeography. The gut microbiota composition and community structure of Bombus pyrosoma from different geographical locations were diverse. On the whole, the gut bacteria Gilliamella and Snodgrassella are dominant in bumblebees, but opportunistic pathogens Serratia and Pseudomonas are dominant in some sampling sites such as Hb15, Gs1, Gs45, Qhs15, and Ssx35. All or part of environmental factors such as latitude, annual mean temperature, elevation, human footprint, population density, and annual precipitation can affect the alpha diversity and community structure of gut bacteria. Further analysis showed that the assembly and shift of bumblebee gut bacterial communities under geographical variation were mainly driven by the stochastic drift of the neutral process rather than by variable selection of niche differentiation. In conclusion, our unprecedented sampling uncovers bumblebee gut microbiome diversity and shifts over evolutionary time.
OBJECTIVE: The microbiotas associated with organisms facilitates host health and fitness, and the homeostasis status of gut microbiota also reflects the habitat security faced by the host. In addition, managing gut microbiota is important to improve bumblebee health by understanding the ecological process of the gut microbiome. Thus, we first carried out an runprecedented sampling of 513 workers of the species Bombus pyrosoma across the Chinese landscape and used full-length 16S rRNA gene sequencing to uncover their gut microbiota diversity and biogeography. Our study provides new insights into the understanding of gut microbiome diversity and shifts for Chinese Bumblebee over evolutionary time.
OBJECTIVE: The microbiotas associated with organisms facilitates host health and fitness, and the homeostasis status of gut microbiota also reflects the habitat security faced by the host. In addition, managing gut microbiota is important to improve bumblebee health by understanding the ecological process of the gut microbiome. Thus, we first carried out an runprecedented sampling of 513 workers of the species Bombus pyrosoma across the Chinese landscape and used full-length 16S rRNA gene sequencing to uncover their gut microbiota diversity and biogeography. Our study provides new insights into the understanding of gut microbiome diversity and shifts for Chinese Bumblebee over evolutionary time.
摘要：
大黄蜂的肠道微生物群至关重要，因为它调节宿主的健康和健身。然而,大黄蜂肠道细菌在长期进化过程中形成和维持多样性的潜在机制尚未阐明。特别是,中国边境的Bombusprosoma的肠道细菌多样性和群落组装过程尚不清楚。在这项研究中,我们系统地对中国各地513名Bombusprosoma物种的工人进行了前所未有的采样，并使用全长16SrRNA基因测序来检查他们的肠道微生物群多样性和生物地理学。来自不同地理位置的Bombusprosoma的肠道微生物组成和群落结构不同。总的来说,肠道细菌Gilliamella和Snodgrassella在大黄蜂中占主导地位，但是在Hb15，Gs1，Gs45，Qhs15和Ssx35等采样点，条件病原体沙雷氏菌和假单胞菌占优势。所有或部分环境因素，如纬度，年平均温度,高程,人类足迹,人口密度，年降水量会影响肠道细菌的α多样性和群落结构。进一步分析表明，地理变异下的大黄蜂肠道细菌群落的组装和迁移主要是由中性过程的随机漂移驱动的，而不是由生态位分化的变量选择驱动的。总之,我们前所未有的采样揭示了大黄蜂肠道微生物组的多样性和随进化时间的变化。
目的：与生物体相关的微生物促进宿主健康和健身，肠道菌群的稳态状态也反映了宿主面临的栖息地安全。此外,通过了解肠道微生物群的生态过程，管理肠道微生物群对于改善大黄蜂健康非常重要。因此，我们首先对中国各地的513名Bombusprosoma物种工人进行了前所未有的采样，并使用全长16SrRNA基因测序来揭示他们的肠道微生物群多样性和生物地理学。我们的研究为理解中国大黄蜂的肠道微生物多样性和进化时间的变化提供了新的见解。
目的：与生物体相关的微生物促进宿主健康和健身，肠道菌群的稳态状态也反映了宿主面临的栖息地安全。此外,通过了解肠道微生物群的生态过程，管理肠道微生物群对于改善大黄蜂健康非常重要。因此，我们首先对中国各地的513名Bombusprosoma物种工人进行了前所未有的采样，并使用全长16SrRNA基因测序来揭示他们的肠道微生物群多样性和生物地理学。我们的研究为理解中国大黄蜂的肠道微生物多样性和进化时间的变化提供了新的见解。
