Abstract：
With implications in several medical conditions, N-linked glycosylation is one of the most important posttranslation modifications present in all living organisms. Due to their nontemplate synthesis, glycan structures are extraordinarily complex and require multiple analytical techniques for complete structural elucidation. Mass spectrometry is the most common way to investigate N-linked glycans; however, with techniques such as liquid-chromatography mass spectrometry, there is complete loss of spatial information. Mass spectrometry imaging is a transformative analytical technique that can visualize the spatial distribution of ions within a biological sample and has been shown to be a powerful tool to investigate N-linked glycosylation. This review covers the fundamentals of mass spectrometry imaging and N-linked glycosylation and highlights important findings of recent key studies aimed at expanding and improving the glycomics imaging field.
摘要：
与几种医疗状况有关，N-连接糖基化是存在于所有活生物体中的最重要的翻译后修饰之一。由于它们的非模板合成，聚糖结构非常复杂，需要多种分析技术才能完成结构阐明。质谱是研究N-连接聚糖的最常用方法；然而，利用液相色谱质谱等技术，空间信息完全丢失。质谱成像是一种转化分析技术，可以可视化生物样品中离子的空间分布，并已被证明是研究N-连接糖基化的强大工具。这篇综述涵盖了质谱成像和N-连接糖基化的基本原理，并强调了旨在扩大和改善糖组学成像领域的最新关键研究的重要发现。
