PDF(Pubmed)
Abstract：
Ginsenoside Rd is a tetracyclic triterpenoid derivative, widely existing in Panax ginseng, Panax notoginseng and other traditional Chinese medicines. Many studies have proved that ginsenoside Rd have a variety of significant biological activities on certain types of cancer. However, the mechanism of ginsenoside Rd remains unclear in lung cancer. The findings of this study reveal that GS-Rd inhibits the proliferation of NSCLC cells, induces apoptosis, and suppresses migration and invasion. The results showed Ginsenoside Rd inhibited the cell proliferation (∼99.52 %) by S phase arrest in cell cycle and promoted the apoptosis (∼54.85 %) of NSCLC cells. It also inhibited the migration and invasion of cells (p < 0.001). The expression levels of related mitochondrial apoptosis proteins (Bax/Bcl-2/Cytochrome C) and matrix metalloproteinases (MMP-2/-9) were significantly changed. The results showed that ginsenoside Rd inhibited the proliferation of tumor cells by activating p53/bax-mediated mitochondrial apoptosis and the expression of key enzymes for cell apoptosis caspase-3/cleaved-caspase-3 were significantly increased. This research contributes to a better understanding of the anti-tumor effects and molecular mechanisms of GS-Rd, paving the way for its potential development and clinical application in NSCLC therapy.
摘要：
人参皂苷Rd是一种四环三萜衍生物，广泛存在于人参中，三七等中药。很多研讨证明人参皂苷Rd对某些类型的癌症具有多种显著的生物学活性。然而,人参皂苷Rd在肺癌中的作用机制尚不清楚。这项研究的结果表明，GS-Rd抑制NSCLC细胞的增殖，诱导细胞凋亡，并抑制迁移和入侵。结果表明，人参皂苷Rd通过细胞周期S期阻滞抑制细胞增殖(~99.52%)，促进NSCLC细胞凋亡(~54.85%)。它还抑制细胞的迁移和侵袭(p<0.001)。线粒体凋亡相关蛋白(Bax/Bcl-2/细胞色素C)和基质金属蛋白酶(MMP-2/-9)的表达水平显著改变。结果表明，人参皂苷Rd通过激活p53/bax介导的线粒体凋亡抑制肿瘤细胞的增殖，细胞凋亡关键酶caspase-3/cleaved-caspase-3的表达明显增加。这项研究有助于更好地了解GS-Rd的抗肿瘤作用和分子机制，为其在NSCLC治疗中的潜在开发和临床应用铺平了道路。
