PDF(Pubmed)
Abstract：
In recent years, burgeoning research has underscored the pivotal role of non-coding RNA in orchestrating the growth, development, and pathogenesis of various diseases across organisms. However, despite these advances, our understanding of the specific contributions of long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) to lens development remains notably limited. Clarifying the intricate gene regulatory networks is imperative for unraveling the molecular underpinnings of lens-related disorders. In this study, we aimed to address this gap by conducting a comprehensive analysis of the expression profiles of messenger RNAs (mRNAs), lncRNAs, and circRNAs at critical developmental time points of the mouse lens, encompassing both embryonic (E10.5, E12.5, and E16.5) and postnatal stages (P0.5, P10.5, and P60). Leveraging RNA-sequencing technology, we identified key transcripts pivotal to lens development. Our analysis revealed differentially expressed (DE) mRNAs, lncRNAs, and circRNAs across various developmental stages. Particularly noteworthy, there were 1831 co-differentially expressed (CO-DE) mRNAs, 150 CO-DE lncRNAs, and 13 CO-DE circRNAs identified during embryonic stages. Gene Ontology (GO) enrichment analysis unveiled associations primarily related to lens development, DNA conformational changes, and angiogenesis among DE mRNAs and lncRNAs. Furthermore, employing protein-protein interaction networks, mRNA-lncRNA co-expression networks, and circRNA-microRNA-mRNA networks, we predicted candidate key molecules implicated in lens development. Our findings underscore the pivotal roles of lncRNAs and circRNAs in this process, offering fresh insights into the pathogenesis of lens-related disorders and paving the way for future exploration in this field.
摘要：
近年来,新兴的研究强调了非编码RNA在协调生长中的关键作用，发展,以及各种疾病在生物体中的发病机理。然而,尽管取得了这些进步，我们对长非编码RNA(lncRNA)和环状RNA(circRNA)对晶状体发育的具体贡献的理解仍然十分有限.澄清复杂的基因调控网络对于解开晶状体相关疾病的分子基础至关重要。在这项研究中,我们旨在通过对信使RNA（mRNA）的表达谱进行全面分析来解决这一差距，lncRNAs,和circRNAs在小鼠晶状体的关键发育时间点，包括胚胎（E10.5，E12.5和E16.5）和出生后阶段（P0.5，P10.5和P60）。利用RNA测序技术，我们确定了对晶状体发育至关重要的关键转录本。我们的分析揭示了差异表达（DE）mRNA，lncRNAs,和circRNAs跨越不同的发育阶段。特别值得注意的是,有1831个共差异表达(CO-DE)mRNA，150CO-DElncRNAs,和13个在胚胎阶段鉴定的CO-DEcircRNAs。基因本体论（GO）富集分析揭示了主要与晶状体发育有关的关联，DNA构象变化,和DEmRNAs和lncRNAs之间的血管生成。此外，采用蛋白质-蛋白质相互作用网络，mRNA-lncRNA共表达网络，和circRNA-microRNA-mRNA网络，我们预测了与晶状体发育有关的候选关键分子.我们的发现强调了lncRNAs和circRNAs在这个过程中的关键作用，为晶状体相关疾病的发病机制提供了新的见解，并为该领域的未来探索铺平了道路。
