PDF(Pubmed)
Abstract：
Alveolar bone defects caused by tooth loss often lead to challenges in implant dentistry, with a need for development of optimal bone biomaterials to predictably rebuild these tissues. To address this problem, we fabricated a novel bone block using platelet-rich fibrin (PRF) and Deproteinized Bovine Bone Mineral (DBBM), and characterized their mechanical and biological properties. The bone block was prepared by mixing DBBM, Liquid-PRF, and Solid-PRF fragments in various combinations as follows: (1) BLOCK-1 made with Solid-PRF fragments + DBBM, (2) BLOCK-2 made with Liquid-PRF + DBBM, (3) BLOCK-3 made with Solid-PRF fragments + Liquid-PRF + DBBM. The time for solidification and the degradation properties were subsequently recorded. Scanning electron microscopy (SEM) and tensile tests were carried out to investigate the microstructure and mechanical properties of each block. The bioactivity of the three groups towards osteoblast differentiation was also evaluated by culturing cells with the conditioned medium from each of the three groups including cell proliferation assay, cell migration assay, alkaline phosphatase (ALP) staining, and alizarin red staining (ARS), as well as by real-time PCR for genes encoding runt-related transcription factor 2 (RUNX2), ALP, collagen type I alpha1(COL1A1) and osteocalcin (OCN). BLOCK-3 made with Solid-PRF fragments + Liquid-PRF + DBBM had by far the fastest solidification period (over a 10-fold increase) as well as the most resistance to degradation. SEM and tensile tests also revealed that the mechanical properties of BLOCK-3 were superior in strength when compared to all other groups and further induced the highest osteoblast migration and osteogenic differentiation confirmed by ALP, ARS and real-time PCR. PRF bone blocks made through the combination of Solid-PRF fragments + Liquid-PRF + DBBM had the greatest mechanical and biological properties when compared to either used alone. Future clinical studies are warranted to further support the clinical application of PRF bone blocks in bone regeneration procedures.
摘要：
牙齿脱落引起的牙槽骨缺损通常会导致种植体牙科面临挑战，需要开发最佳的骨生物材料来可预测地重建这些组织。为了解决这个问题,我们使用富血小板纤维蛋白（PRF）和去蛋白牛骨矿物质（DBBM）制造了一种新型骨块，并表征了它们的机械和生物学特性。通过混合DBBM制备骨块，Liquid-PRF,和Solid-PRF片段的各种组合如下:(1)用Solid-PRF片段+DBBM制备的BLOCK-1,（2）用液体PRF+DBBM制成的BLOCK-2，(3)用固体-PRF碎片+液体-PRF+DBBM制备的BLOCK-3。随后记录固化的时间和降解性质。进行了扫描电子显微镜（SEM）和拉伸测试，以研究每个块的微观结构和力学性能。还通过用来自三组中的每一组的条件培养基培养细胞来评估三组对成骨细胞分化的生物活性，包括细胞增殖测定。细胞迁移试验，碱性磷酸酶(ALP)染色,和茜素红染色（ARS），以及通过实时PCR检测编码runt相关转录因子2（RUNX2）的基因，ALP,I型胶原α1（COL1A1）和骨钙蛋白（OCN）。用固体-PRF碎片+液体-PRF+DBBM制成的BLOCK-3具有迄今为止最快的固化期(超过10倍的增加)以及对降解的抗性。SEM和拉伸试验还显示，与所有其他组相比，BLOCK-3的机械性能在强度上更优越，并进一步诱导了ALP证实的最高成骨细胞迁移和成骨分化。ARS和实时PCR。通过固体-PRF碎片+液体-PRF+DBBM的组合制成的PRF骨块与单独使用的任一种相比具有最大的机械和生物学特性。未来的临床研究有必要进一步支持PRF骨块在骨再生手术中的临床应用。
