Abstract:
A putative glufosinate-resistant Amaranthus palmeri population was reported in 2015 in Anson County, North Carolina. The results from dose-response assays conducted in the field suggested plants were surviving lethal rates of glufosinate. Dose-response assays conducted in the glasshouse determined the Anson County accession exhibited reduced susceptibility to glufosinate compared to three glufosinate-susceptible populations. The LD50 values (210-316 g ai ha-1) for the Anson County population were always higher than the LD50 values (118-158 g ai ha-1) for the tested susceptible populations from the dose-response assays. Anson County plants that survived lethal glufosinate rates were reciprocally crossed with susceptible plants to create F1 genotypes and treated with a lethal rate of glufosinate (267 g ai ha-1; ascertained from glasshouse dose-response assay) to determine the distribution of injury and survival for each cross compared to a cross of susceptible parents. The distribution of injury was non-normal for the crosses containing an Anson County plant compared to the cross with a susceptible parent. Survival was 68%-84% for crosses containing an Anson County plant, whereas the survival was significantly reduced to 35% for the susceptible plant cross. Chi-square goodness of fit tests were used to test inheritance models to describe the responses of the genotypes. The resistant × susceptible crosses were best described with a heterozygous two loci with incomplete dominance model compared to the resistant × resistant cross that was best described with a heterozygous single locus with incomplete dominance model. The Anson County population has evolved resistance to glufosinate that is heritable and likely conferred by an oligogenic mechanism with incomplete dominance.
摘要:
2015年,安森县报告了推定的耐草铵膦的Amaranthuspalmeri种群,北卡罗来纳州。在田间进行的剂量反应测定的结果表明,植物存活的草铵膦致死率。在温室中进行的剂量反应测定确定,与三个草铵膦易感人群相比,安森县的登录名对草铵膦的易感性降低。安森县人群的LD50值(210-316gaiha-1)始终高于剂量反应测定中测试的易感人群的LD50值(118-158gaiha-1)。将存活致死草铵膦率的安森县植物与易感植物相互杂交,以创建F1基因型,并用草铵膦的致死率(267gaiha-1;从温室剂量反应测定中确定)进行处理,以确定与易感亲本的杂交相比,每个杂交的损伤和存活分布。与易感亲本的杂交相比,包含安森县植物的杂交的伤害分布不正常。包含安森县植物的杂交存活率为68%-84%,而易感植物杂交的存活率显着降低至35%。卡方拟合优度检验用于测试遗传模型以描述基因型的反应。与具有不完全显性模型的杂合单个基因座最好描述的抗性×抗性杂交相比,抗性×易感杂交用具有不完全显性模型的杂合两个基因座最好描述。安森县的人口已经对草铵膦产生了抗性,这种抗性是可遗传的,并且可能是由具有不完全优势的寡基因机制赋予的。