PDF(Pubmed)
Abstract：
Cancer is one of the most challenging diseases in the world. Recently, iron oxide nanoparticles (IONPs) are emerging materials with rapid development and high application value, and have shown great potential on tumor therapy due to their unique magnetic and biocompatible properties. However, some data hint us that IONPs were toxic to normal cells and vital organs. Thus, more data on biosafety evaluation is urgently needed. In this study, we compared the effects of silicon-coated IONPs (Si-IONPs) on two cell types: the tumor cells (Hela) and the normal cells (HEK293T, as 293 T for short), compared differences of protein composition, allocation and physical characteristics between these two cells. The major findings of our study pointed out that 293 T cells death occurred more significant than that of Hela cells after Si-IONPs treatment, and the rate and content of endocytosis of Si-IONPs in 293 T cells was more prominent than in Hela cells. Our results also showed Si-IONPs significant promoted the production of reactive oxygen species and disturbed pathways related to oxidative stress, iron homeostasis, apoptosis and ferroptosis in both two types of cells, however, Hela cells recovered from these disturbances more easily than 293 T. In conclusion, compared with Hela cells, IONPs are more likely to induce 293 T cells death and Hela cells have their own unique mechanisms to defense invaders, reminding scientists that future in vivo and in vitro studies of nanoparticles need to be cautious, and more safety data are needed for further clinical treatment.
摘要：
癌症是世界上最具挑战性的疾病之一。最近,纳米氧化铁(IONP)是发展迅速、应用价值高的新兴材料,由于其独特的磁性和生物相容性，在肿瘤治疗中显示出巨大的潜力。然而,一些数据提示IONP对正常细胞和重要器官具有毒性。因此，迫切需要更多关于生物安全性评估的数据。在这项研究中,我们比较了硅涂层IONP（Si-IONP）对两种细胞类型的影响：肿瘤细胞（Hela）和正常细胞（HEK293T，简称293T)，比较蛋白质组成的差异，这两个细胞之间的分配和物理特征。我们研究的主要发现指出，在Si-IONPs处理后，293T细胞的死亡发生比Hela细胞更显著，293T细胞中Si-IONPs的内吞速率和含量明显高于Hela细胞。我们的结果还表明，Si-IONP显著促进活性氧的产生和与氧化应激相关的干扰途径，铁稳态，两种类型细胞的凋亡和铁凋亡，然而,Hela细胞比293T更容易从这些干扰中恢复。与Hela细胞相比，IONP更有可能诱导293T细胞死亡，Hela细胞有自己独特的机制来防御入侵者，提醒科学家，未来对纳米粒子的体内和体外研究需要谨慎，进一步的临床治疗需要更多的安全性数据.
