PDF(Pubmed)
Abstract：
UNASSIGNED: Aging is the most significant contributor to the increasing prevalence of atrial fibrillation (AF). Dysbiosis of gut microbiota has been implicated in age-related diseases, but its role in AF development remains unclear. This study aimed to investigate the correlations between changes in the autonomic nervous system, short-chain fatty acids (SCFAs), and alterations in gut microbiota in aged rats with AF.
UNASSIGNED: Electrophysiological experiments were conducted to assess AF induction rates and heart rate variability in rats. 16S rRNA gene sequences extracted from fecal samples were used to assess the gut microbial composition. Gas and liquid chromatography-mass spectroscopy was used to identify SCFAs in fecal samples.
UNASSIGNED: The study found that aged rats exhibited a higher incidence of AF and reduced heart rate variability compared to young rats. Omics research revealed disrupted gut microbiota in aged rats, specifically a decreased Firmicutes to Bacteroidetes ratio. Additionally, fecal SCFA levels were significantly lower in aged rats. Importantly, correlation analysis indicated a significant association between decreased SCFAs and declining heart rate variability in aged rats.
UNASSIGNED: These findings suggest that SCFAs, as metabolites of gut microbiota, may play a regulatory role in autonomic nervous function and potentially influence the onset and progression of AF in aged rats. These results provide novel insights into the involvement of SCFAs and autonomic nervous system function in the pathogenesis of AF. These results provide novel insights into the involvement of SCFAs and autonomic nervous system function in the pathogenesis of AF.
UNASSIGNED: Electrophysiological experiments were conducted to assess AF induction rates and heart rate variability in rats. 16S rRNA gene sequences extracted from fecal samples were used to assess the gut microbial composition. Gas and liquid chromatography-mass spectroscopy was used to identify SCFAs in fecal samples.
UNASSIGNED: The study found that aged rats exhibited a higher incidence of AF and reduced heart rate variability compared to young rats. Omics research revealed disrupted gut microbiota in aged rats, specifically a decreased Firmicutes to Bacteroidetes ratio. Additionally, fecal SCFA levels were significantly lower in aged rats. Importantly, correlation analysis indicated a significant association between decreased SCFAs and declining heart rate variability in aged rats.
UNASSIGNED: These findings suggest that SCFAs, as metabolites of gut microbiota, may play a regulatory role in autonomic nervous function and potentially influence the onset and progression of AF in aged rats. These results provide novel insights into the involvement of SCFAs and autonomic nervous system function in the pathogenesis of AF. These results provide novel insights into the involvement of SCFAs and autonomic nervous system function in the pathogenesis of AF.
摘要：
■衰老是导致心房颤动（AF）患病率增加的最重要因素。肠道微生物群的菌群失调与年龄相关的疾病有关，但其在房颤发展中的作用尚不清楚。本研究旨在探讨自主神经系统变化之间的相关性。短链脂肪酸（SCFA），老年房颤大鼠肠道菌群的变化。
■进行电生理实验以评估大鼠的AF诱导率和心率变异性。从粪便样品中提取的16SrRNA基因序列用于评估肠道微生物组成。使用气相和液相色谱-质谱来鉴定粪便样品中的SCFA。
■研究发现，与年轻大鼠相比，老年大鼠的AF发生率更高，心率变异性降低。组学研究显示，老年大鼠的肠道微生物群遭到破坏，特别是Firmicutes与拟杆菌的比率降低。此外,老年大鼠粪便SCFA水平明显降低。重要的是,相关分析表明，老年大鼠SCFA降低与心率变异性降低之间存在显着关联。
■这些发现表明SCFA，作为肠道微生物群的代谢产物，可能在植物神经功能中起调节作用，并可能影响老年大鼠AF的发生和进展。这些结果为SCFA和自主神经系统功能在AF发病机理中的参与提供了新的见解。这些结果为SCFA和自主神经系统功能在AF发病机理中的参与提供了新的见解。
■进行电生理实验以评估大鼠的AF诱导率和心率变异性。从粪便样品中提取的16SrRNA基因序列用于评估肠道微生物组成。使用气相和液相色谱-质谱来鉴定粪便样品中的SCFA。
■研究发现，与年轻大鼠相比，老年大鼠的AF发生率更高，心率变异性降低。组学研究显示，老年大鼠的肠道微生物群遭到破坏，特别是Firmicutes与拟杆菌的比率降低。此外,老年大鼠粪便SCFA水平明显降低。重要的是,相关分析表明，老年大鼠SCFA降低与心率变异性降低之间存在显着关联。
■这些发现表明SCFA，作为肠道微生物群的代谢产物，可能在植物神经功能中起调节作用，并可能影响老年大鼠AF的发生和进展。这些结果为SCFA和自主神经系统功能在AF发病机理中的参与提供了新的见解。这些结果为SCFA和自主神经系统功能在AF发病机理中的参与提供了新的见解。
