Abstract：
Antibiotic residues may be present in fruit products from trees that were treated to combat bacterial diseases such as citrus greening or blight. A liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method was developed for the simultaneous determination and identification of streptomycin, kasugamycin, penicillin, and oxytetracycline residues in fruit. Samples were extracted with acidic methanol and separation was optimized for a hydrophilic interaction LC column. A Q-Exactive HRMS instrument was used to obtain product ion spectra for analyte identification. Quantitation was performed with matrix-extracted calibration curves and internal standard correction. The method was tested on many different types of fruit. In general, fortified samples demonstrated acceptable recoveries (82-116%) and reproducibility (<15% RSD). Method detection limits for these analytes were well below the established US EPA tolerance levels. It was also possible to analyze the fruit extracts prepared using this method for additional chemical contaminants using LC-HRMS.
摘要：
抗生素残留物可能存在于经过处理以对抗细菌性疾病如柑橘绿化或枯萎病的树木的水果产品中。建立了同时测定和鉴定链霉素的液相色谱-高分辨质谱(LC-HRMS)方法,卡苏霉素,青霉素,和土霉素在水果中的残留。用酸性甲醇提取样品,并针对亲水相互作用LC柱优化分离。Q-ExactiveHRMS仪器用于获得产物离子光谱以用于分析物鉴定。用基质提取的校准曲线和内标校正进行定量。在许多不同类型的水果上测试了该方法。总的来说,强化样品表现出可接受的回收率(82-116%)和重现性(<15%RSD)。这些分析物的方法检测极限远低于已建立的USEPA耐受水平。还可以使用LC-HRMS分析使用该方法制备的果实提取物中的其他化学污染物。
