Abstract：
OBJECTIVE: We investigated the relationship of circulating TSP-1 mRNA and miR-194 with diabetic kidney disease\'s degree.
METHODS: We enrolled 167 hospitalized type 2 diabetes patients in the endocrinology department. Patients were split into three groups according to urinary microalbumin: A, B and C. The control group comprised healthy outpatients (n = 163). The quantities of microribonucleic acid (miR)-194 and thrombospondin-1 (TSP-1) messenger ribonucleic acid (mRNA) in the participants\' circulation were measured using a quantitative real-time polymerase chain reaction.
RESULTS: Circulating TSP-1 mRNA (P = 0.024) and miR-194 (P = 0.029) expressions significantly increased in type 2 diabetes patients. Circulating TSP-1 mRNA (P = 0.040) and miR-194 (P = 0.007) expression levels differed significantly among the three groups; circulating TSP-1 mRNA expression increased with urinary microalbumin. However, miR-194 declined in group B and increased in group C. Circulating TSP-1 mRNA was positively correlated with cystatin-c (r = 0.281; P = 0.021) and microalbumin/creatinine ratio (UmALB/Cr; r = 0.317; P = 0.009); miR-194 was positively correlated with UmALB/Cr (r = 0.405; P = 0.003). Stepwise multivariate linear regression analysis showed cystatin-c (β = 0.578; P = 0.021) and UmALB/Cr (β = 0.001; P = 0.009) as independent factors for TSP-1 mRNA; UmALB/Cr (β = 0.005; P = 0.028) as an independent factor for miR194. Areas under the curve for circulating TSP-1 mRNA and miR194 were 0.756 (95% confidence interval 0.620-0.893; sensitivity 0.69 and specificity 0.71, P < 0.01) and 0.584 (95% confidence interval 0.421-0.748; sensitivity 0.54 and specificity 0.52, P < 0.01), respectively.
CONCLUSIONS: Circulating TSP-1 mRNA and miR-194 expressions significantly increased in type 2 diabetes patients. The microalbumin group had lower levels of miR-194 (a risk factor that is valuable for type 2 diabetes kidney disease evaluation).
METHODS: We enrolled 167 hospitalized type 2 diabetes patients in the endocrinology department. Patients were split into three groups according to urinary microalbumin: A, B and C. The control group comprised healthy outpatients (n = 163). The quantities of microribonucleic acid (miR)-194 and thrombospondin-1 (TSP-1) messenger ribonucleic acid (mRNA) in the participants\' circulation were measured using a quantitative real-time polymerase chain reaction.
RESULTS: Circulating TSP-1 mRNA (P = 0.024) and miR-194 (P = 0.029) expressions significantly increased in type 2 diabetes patients. Circulating TSP-1 mRNA (P = 0.040) and miR-194 (P = 0.007) expression levels differed significantly among the three groups; circulating TSP-1 mRNA expression increased with urinary microalbumin. However, miR-194 declined in group B and increased in group C. Circulating TSP-1 mRNA was positively correlated with cystatin-c (r = 0.281; P = 0.021) and microalbumin/creatinine ratio (UmALB/Cr; r = 0.317; P = 0.009); miR-194 was positively correlated with UmALB/Cr (r = 0.405; P = 0.003). Stepwise multivariate linear regression analysis showed cystatin-c (β = 0.578; P = 0.021) and UmALB/Cr (β = 0.001; P = 0.009) as independent factors for TSP-1 mRNA; UmALB/Cr (β = 0.005; P = 0.028) as an independent factor for miR194. Areas under the curve for circulating TSP-1 mRNA and miR194 were 0.756 (95% confidence interval 0.620-0.893; sensitivity 0.69 and specificity 0.71, P < 0.01) and 0.584 (95% confidence interval 0.421-0.748; sensitivity 0.54 and specificity 0.52, P < 0.01), respectively.
CONCLUSIONS: Circulating TSP-1 mRNA and miR-194 expressions significantly increased in type 2 diabetes patients. The microalbumin group had lower levels of miR-194 (a risk factor that is valuable for type 2 diabetes kidney disease evaluation).
摘要：
目的：我们研究了循环TSP-1mRNA和miR-194与糖尿病肾病程度的关系。
方法：我们在内分泌科招募了167名住院的2型糖尿病患者。根据尿微量白蛋白将患者分为三组：A，B和C.对照组包括健康门诊患者(n=163)。使用定量实时聚合酶链反应测量参与者循环中的微核糖核酸（miR）-194和血小板反应蛋白-1（TSP-1）信使核糖核酸（mRNA）的数量。
结果：在2型糖尿病患者中，循环TSP-1mRNA（P=0.024）和miR-194（P=0.029）表达显著增加。循环TSP-1mRNA(P=0.040)和miR-194(P=0.007)表达水平在三组间有显著差异；循环TSP-1mRNA表达水平随尿微量白蛋白的增加而增加。然而,miR-194在B组下降，C组升高。循环TSP-1mRNA与胱抑素c（r=0.281；P=0.021）和微量白蛋白/肌酐比值（UmALB/Cr；r=0.317；P=0.009）呈正相关；miR-194与UmALB/Cr呈正相关（r=0.405；P=0.003）。多元线性回归分析显示,胱抑素c(β=0.578;P=0.021)和UmALB/Cr(β=0.001;P=0.009)为TSP-1mRNA的独立影响因素,UmALB/Cr(β=0.005;P=0.028)为miR194的独立影响因素。循环TSP-1mRNA和miR194的曲线下面积分别为0.756（95％置信区间0.620-0.893；敏感性0.69和特异性0.71，P<0.01）和0.584（95％置信区间0.421-0.748；敏感性0.54和特异性0.52，P<0.01）。分别。
结论：在2型糖尿病患者中循环TSP-1mRNA和miR-194表达显著增加。微量白蛋白组的miR-194水平较低（对2型糖尿病肾病评估有价值的危险因素）。
方法：我们在内分泌科招募了167名住院的2型糖尿病患者。根据尿微量白蛋白将患者分为三组：A，B和C.对照组包括健康门诊患者(n=163)。使用定量实时聚合酶链反应测量参与者循环中的微核糖核酸（miR）-194和血小板反应蛋白-1（TSP-1）信使核糖核酸（mRNA）的数量。
结果：在2型糖尿病患者中，循环TSP-1mRNA（P=0.024）和miR-194（P=0.029）表达显著增加。循环TSP-1mRNA(P=0.040)和miR-194(P=0.007)表达水平在三组间有显著差异；循环TSP-1mRNA表达水平随尿微量白蛋白的增加而增加。然而,miR-194在B组下降，C组升高。循环TSP-1mRNA与胱抑素c（r=0.281；P=0.021）和微量白蛋白/肌酐比值（UmALB/Cr；r=0.317；P=0.009）呈正相关；miR-194与UmALB/Cr呈正相关（r=0.405；P=0.003）。多元线性回归分析显示,胱抑素c(β=0.578;P=0.021)和UmALB/Cr(β=0.001;P=0.009)为TSP-1mRNA的独立影响因素,UmALB/Cr(β=0.005;P=0.028)为miR194的独立影响因素。循环TSP-1mRNA和miR194的曲线下面积分别为0.756（95％置信区间0.620-0.893；敏感性0.69和特异性0.71，P<0.01）和0.584（95％置信区间0.421-0.748；敏感性0.54和特异性0.52，P<0.01）。分别。
结论：在2型糖尿病患者中循环TSP-1mRNA和miR-194表达显著增加。微量白蛋白组的miR-194水平较低（对2型糖尿病肾病评估有价值的危险因素）。
