PDF(Pubmed)
Abstract：
Classical swine fever (CSF), caused by the classical swine fever virus (CSFV), results in significant economic losses to the swine industry in many countries. Vaccination represents the primary strategy to control CSF and the CSFV E2 protein is known as the major protective antigen. However, the E2 protein expressed or presented by different systems elicits distinct immune responses. In this study, we established a stable CHO cell line to express the E2 protein and delivered it using self-assembled ferritin nanoparticles (NPs). Subsequently, we compared the adaptive immune responses induced by the E2-ferritin NPs and the monomeric E2 protein produced by the CHO cells or a baculovirus expression system. The results revealed that the NP-delivered E2 protein elicited higher titers of neutralizing antibodies than did the monomeric E2 protein in pigs. Importantly, only the NP-delivered E2 protein significantly induced CSFV-specific IFN-γ-secreting cells. Furthermore, all the pigs inoculated with the E2-ferritin NPs were completely protected from a lethal CSFV challenge infection. These findings demonstrate the ability of the E2-ferritin NPs to protect pigs against the lethal CSFV challenge by eliciting robust humoral and cellular immune responses.
摘要：
猪瘟(CSF),由经典猪瘟病毒（CSFV）引起，给许多国家的养猪业造成了巨大的经济损失。疫苗接种代表控制CSF的主要策略,并且已知CSFVE2蛋白是主要的保护性抗原。然而,由不同系统表达或呈递的E2蛋白引发不同的免疫应答。在这项研究中,我们建立了稳定的CHO细胞系来表达E2蛋白，并使用自组装的铁蛋白纳米颗粒（NP）将其递送。随后，我们比较了E2-铁蛋白NP和CHO细胞或杆状病毒表达系统产生的单体E2蛋白诱导的适应性免疫应答.结果显示,NP递送的E2蛋白在猪中比单体E2蛋白引发更高滴度的中和抗体。重要的是,只有NP递送的E2蛋白显着诱导CSFV特异性IFN-γ分泌细胞。此外，所有接种E2-铁蛋白NPs的猪完全免受致命的CSFV攻击感染。这些发现证明了E2-铁蛋白NP通过引发强大的体液和细胞免疫应答来保护猪免受致命CSFV攻击的能力。
