PDF(Pubmed)
Abstract：
In the current study, a novel strain of Fusarium oxysporum alternavirus 1 (FoAV1) was identified from the Fusarium oxysporum f. sp. melonis (FOM) strain T-BJ17 and was designated as Fusarium oxysporum alternavirus 1-FOM (FoAV1-FOM). Its genome consists of four dsRNA segments of 3515 bp (dsRNA1), 2663 bp (dsRNA2), 2368 bp (dsRNA3), and 1776 bp (dsRNA4) in length. Open reading frame 1 (ORF1) in dsRNA1 was found to encode a putative RNA-dependent RNA polymerase (RdRp), whose amino acid sequence was 99.02% identical to that of its counterpart in FoAV1; while ORF2 in dsRNA2, ORF3 in dsRNA3, and ORF4 in dsRNA4 were all found to encode hypothetical proteins. Strain T-BJ17-VF, which was verified to FoAV1-FOM-free, was obtained using single-hyphal-tip culture combined with high-temperature treatment to eliminate FoAV1-FOM from strain T-BJ17. The colony growth rate, ability to produce spores, and virulence of strain T-BJ17 were significantly lower than those of T-BJ17-VF, while the dry weight of the mycelial biomass and the sensitivity to difenoconazole and pydiflumetofen of strain T-BJ17 were greater than those of T-BJ17-VF. FoAV1-FOM was capable of 100% vertical transmission via spores. To our knowledge, this is the first time that an alternavirus has infected FOM, and this is the first report of hypovirulence and increased sensitivity to difenoconazole and pydiflumetofen induced by FoAV1-FOM infection in FOM.
摘要：
在目前的研究中,从尖孢镰刀菌f.sp.中鉴定出一种新型的尖孢镰刀菌交替病毒1（FoAV1）。甜瓜(FOM)株T-BJ17,并被指定为尖孢镰刀菌交替病毒1-FOM(FoAV1-FOM)。它的基因组由四个3515bp的dsRNA片段(dsRNA1)组成，2663bp（dsRNA2），2368bp（dsRNA3），和1776bp（dsRNA4）的长度。dsRNA1中的开放阅读框1（ORF1）被发现编码推定的RNA依赖性RNA聚合酶（RdRp），其氨基酸序列与FoAV1中的氨基酸序列99.02％相同；而dsRNA2中的ORF2，dsRNA3中的ORF3和dsRNA4中的ORF4均被发现编码假设的蛋白质。应变T-BJ17-VF,这被验证为无FoAV1-FOM，使用单菌丝尖端培养结合高温处理从菌株T-BJ17中消除FoAV1-FOM获得。菌落生长速度,产生孢子的能力，毒力T-BJ17显著低于T-BJ17-VF,而菌株T-BJ17的菌丝体生物量干重和对苯醚甲环唑和吡氟美芬的敏感性大于T-BJ17-VF。FoAV1-FOM能够通过孢子100%垂直传播。据我们所知,这是第一次交替病毒感染FOM，这是FoAV1-FOM感染引起的低毒力和对苯醚甲环唑和吡氟美芬敏感性增加的第一份报告。
