PDF(Pubmed)
Abstract：
Congenital cytomegalovirus (cCMV) infection poses significant risks to fetal development, particularly affecting the nervous system. This study reports a fetal autopsy case, examining cCMV infection and focusing on CMV DNA measurements in various fetal organs before formalin fixation, a novel approach for comprehensive CMV DNA evaluations in fetal organs affected by cCMV. A 20-week-old male fetus was diagnosed with cCMV following the detection of CMV DNA in ascites obtained via abdominocentesis in utero. After the termination of pregnancy, multiple organs of the fetus, including the cerebrum, thyroid gland, heart, lungs, liver, spleen, kidneys, and adrenal glands, were extracted and examined for CMV DNA loads using a real-time polymerase chain reaction. Histopathological examination involved hematoxylin-eosin and CMV-specific immunostaining. A correlation was found between CMV DNA loads and pathology, with higher CMV-infected cell numbers observed in organs positively identified with both staining methods, exhibiting CMV DNA levels of ≥1.0 × 104 copies/mL, compared to those detected solely by CMV-specific immunostaining, where CMV DNA levels ranged from 1.0 × 103 to 1.0 × 104 copies/mL. These results highlight a quantifiable relationship between the organ infection extent and CMV DNA concentration, providing insights into cCMV pathogenesis and potentially informing future diagnostic and therapeutic strategies for cCMV infection.
摘要：
先天性巨细胞病毒（cCMV）感染对胎儿发育构成重大风险，尤其影响神经系统。这项研究报告了一个胎儿尸检案例，在福尔马林固定之前检查cCMV感染并关注各种胎儿器官的CMVDNA测量，一种在受cCMV影响的胎儿器官中全面评估CMVDNA的新方法。在通过子宫腹腔穿刺术获得的腹水中检测到CMVDNA后，一名20周大的男性胎儿被诊断为cCMV。终止妊娠后,胎儿的多个器官，包括大脑,甲状腺,心,肺,肝脏,脾,脾肾脏，和肾上腺，提取并使用实时聚合酶链反应检查CMVDNA负载。组织病理学检查涉及苏木精-伊红和CMV特异性免疫染色。CMVDNA载量与病理之间存在相关性，在两种染色方法阳性鉴定的器官中观察到更高的CMV感染细胞数量，表现出CMVDNA水平≥1.0×104拷贝/mL，与仅通过CMV特异性免疫染色检测到的相比，其中CMVDNA水平范围为1.0×103至1.0×104拷贝/mL。这些结果突出了器官感染程度和CMVDNA浓度之间的可量化关系，提供对cCMV发病机制的见解，并可能为cCMV感染的未来诊断和治疗策略提供信息。
