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Abstract：
A rapid and online microvolume flow-through dialysis probe designed for sample preparation in the analysis of veterinary drug residues is introduced. This study addresses the need for efficient and green sample preparation methods that reduce chemical waste and reagent use. The dialysis probe integrates with liquid chromatography and mass spectrometry (LC-MS) systems, facilitating automated, high-throughput analysis. The dialysis method utilizes minimal reagent volumes per sample, significantly reducing the generation of solvent waste compared to traditional sample preparation techniques. Several veterinary drugs were spiked into tissue homogenates and analyzed to validate the probe\'s efficacy. A diagnostic sensitivity of >97% and specificity of >95% were obtained for this performance evaluation. The results demonstrated the effective removal of cellular debris and particulates, ensuring sample integrity and preventing instrument clogging. The automated dialysis probe yielded recovery rates between 27 and 77% for multiple analytes, confirming its potential to streamline veterinary drug residue analysis, while adhering to green chemistry principles. The approach highlights substantial improvements in both environmental impact and operational efficiency, presenting a viable alternative to conventional sample preparation methods in regulatory and research applications.
摘要：
介绍了一种设计用于兽药残留分析中样品制备的快速在线微体积流通透析探针。这项研究解决了对减少化学废物和试剂使用的高效绿色样品制备方法的需求。透析探头与液相色谱和质谱（LC-MS）系统集成，促进自动化，高通量分析。透析方法利用每个样品的最小试剂体积，与传统的样品制备技术相比，显著减少了溶剂废物的产生。将几种兽药掺入组织匀浆中并进行分析以验证探针的功效。对于该性能评估,获得>97%的诊断灵敏度和>95%的特异性。结果表明有效去除细胞碎片和颗粒，确保样品的完整性和防止仪器堵塞。自动透析探针对多种分析物的回收率在27％至77％之间，确认其简化兽药残留分析的潜力，同时坚持绿色化学原则。该方法突出了环境影响和运营效率的实质性改进，在监管和研究应用中提出了传统样品制备方法的可行替代方案。
