PDF(Pubmed)
Abstract：
We present the design, fabrication, and testing of a low-cost, miniaturized detection system that utilizes chemiluminescence to measure the presence of adenosine triphosphate (ATP), the energy unit in biological systems, in water samples. The ATP-luciferin chemiluminescent solution was faced to a silicon photomultiplier (SiPM) for highly sensitive real-time detection. This system can detect ATP concentrations as low as 0.2 nM, with a sensitivity of 79.5 A/M. Additionally, it offers rapid response times and can measure the characteristic time required for reactant diffusion and mixing within the reaction volume, determined to be 0.3 ± 0.1 s. This corresponds to a diffusion velocity of approximately 44 ± 14 mm2/s.
摘要：
我们提出的设计，fabrication,和低成本的测试，小型化检测系统，利用化学发光来测量三磷酸腺苷（ATP）的存在，生物系统中的能量单位，在水样中。ATP-荧光素化学发光溶液面对硅光电倍增管（SiPM），以进行高灵敏度的实时检测。该系统可以检测低至0.2nM的ATP浓度，灵敏度为79.5A/M。此外,它提供了快速的响应时间，并可以测量反应体积内反应物扩散和混合所需的特征时间，确定为0.3±0.1s。这对应于大约44±14mm2/s的扩散速度。
