PDF(Pubmed)
Abstract：
Ethanol extracts obtained from 13 poplar propolis samples originating from various European countries by traditional maceration were tested for total polyphenols, flavonoid content, and antioxidant activity. Moreover, the content of 18 polyphenolic compounds (from the group of phenolic acids and flavonoids) was determined using the HPLC method. The inhibitory effect of six selected extracts with the highest activity was assessed by well-diffusion method against five strains (Bifidobacterium spp., L. rhamnosus, L. acidophilus, E. coli, and Bacteroides spp.) of intestinal bacteria self-isolated from the faeces of obese probands with the use of selective media. It was found that the antioxidant activity of propolis varied depending on geographical origin and even among samples from the same region, which indicates that some other factors also influence propolis quality. The samples of different geographical origin varied mainly in the share of individual phenolic compounds, and it was not possible to find a characteristic marker of origin, excluding the galangin present in the Polish samples only. Assessing the inhibitory activity of propolis (in the range of 70 mg to 10 µg per mL) indicated that the concentration of 100 µg/mL was found as being safe for tested fecal bacteria (Bifidobacterium spp., L. rhamnosus, L. acidophilus, E. coli, and Bacteroides spp.). As no negative effect of low doses of propolis on the intestinal microflora was found, it can be suggested that its use in recommended doses brings only beneficial effects to the body.
摘要：
通过传统浸渍从来自欧洲各个国家的13个杨树蜂胶样品中获得的乙醇提取物进行了总多酚测试，类黄酮含量，和抗氧化活性。此外，用高效液相色谱法测定了18种多酚类化合物（酚酸类和黄酮类化合物）的含量。通过良好扩散法评估了六种具有最高活性的选定提取物对五种菌株的抑制作用（双歧杆菌属。,L.鼠李糖，L.嗜酸菌，大肠杆菌，和拟杆菌属。）使用选择性培养基从肥胖先证者的粪便中自我分离的肠道细菌。发现蜂胶的抗氧化活性因地理来源而异，甚至在同一地区的样品中也有所不同。这表明其他一些因素也会影响蜂胶的质量。不同地理来源的样品主要在单个酚类化合物的份额上有所不同，不可能找到一个特征性的起源标记，仅排除波兰样品中存在的高良姜素。评估蜂胶的抑制活性（在每毫升70mg至10µg的范围内）表明，发现100µg/mL的浓度对于测试的粪便细菌是安全的（双歧杆菌属。,L.鼠李糖，L.嗜酸菌，大肠杆菌，和拟杆菌属。).由于没有发现低剂量蜂胶对肠道菌群的负面影响，可以建议，以推荐剂量使用它只会给身体带来有益的影响。
