PDF(Pubmed)
Abstract：
Azorella compacta (A. compacta) is a shrub of the Andean Altiplano of Bolivia, Chile and Peru, consumed by local communities as a traditional medicine for several maladies such as diabetes, hepatic and inflammatory diseases. A. compacta is rich in mulinane- and azorellane-type diterpenoids. For two of these, acute hypoglycemic effects have been described, but the impact of A. compacta diterpenoids on fatty liver disease has not been investigated. Therefore, A. compacta organic fractions were prepared using petroleum ether, dichloromethane and methanol. Their content was characterized by UHPLC/MS, revealing the presence of ten diterpenoids, mainly mulinic acid, azorellanol and mulin-11,13-diene. Next, mice fed with a high-fat diet (HFD), a model of metabolic dysfunction-associated fatty liver disease (MAFLD), received one of the fractions in drinking water for two weeks. After this treatment, hepatic parameters were evaluated. The A. compacta fractions did not reduce hyperglycemia or body weight in the HFD-fed mice but increased the serum levels of hepatic transaminases (AST and ALT), reduced albumin and increased bilirubin, indicating hepatic damage, while histopathological alterations such as steatosis, inflammation and necrosis generated by the HFD were, overall, not ameliorated by the fractions. These results suggest that organic A. compacta extracts may generate hepatic complications in patients with MAFLD.
摘要：
亚速拉紧凑(A.compacta）是玻利维亚安第斯高原的灌木，智利和秘鲁，被当地社区用作治疗糖尿病等多种疾病的传统医学，肝脏和炎性疾病。A.compacta富含木胺和azorellane型二萜。对于其中两个，已经描述了急性降血糖作用，但是，尚未研究过密参二萜对脂肪肝的影响。因此,A.使用石油醚制备致密有机馏分，二氯甲烷和甲醇。用UHPLC/MS表征其含量，揭示了十种二萜的存在，主要是多元酸，阿唑列醇和木素-11,13-二烯。接下来,高脂肪饮食（HFD）的小鼠，代谢功能障碍相关脂肪性肝病（MAFLD）模型，在饮用水中接受了其中一种成分两周。经过这种治疗,评估肝脏参数。在HFD饲喂的小鼠中,致密A.compacta组分没有降低高血糖或体重,但增加了肝转氨酶(AST和ALT)的血清水平,白蛋白减少和胆红素增加，表明肝损伤，而组织病理学改变，如脂肪变性，HFD产生的炎症和坏死，总的来说,没有被分数改善。这些结果表明，有机A提取物可能会在MAFLD患者中产生肝脏并发症。
