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Abstract：
Hymedesmiidae is one of the largest families of marine sponges and stands out as an exceptional source of variable metabolites with diverse biological activities. In this study, the ethyl acetate fraction (HE) of a Hymedesmia sp. marine sponge from the Red Sea, Egypt, was analyzed for the first time using Ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) analysis. The analysis tentatively identified 29 compounds in this fraction, including the isolation and identification of six compounds (two pyrimidine nucleosides, one purine, and two pyrimidine bases in addition to one cerebroside) for the first time. The structures of the isolated compounds were established by 1D and 2D NMR (nuclear magnetic resonance), MS (mass spectrometry), and IR (infrared) spectroscopy. Furthermore, the cytotoxic, antioxidant, and antimicrobial activities of the ethyl acetate fraction were evaluated in vitro. The fraction exhibited strong DPPH scavenging activity with an IC50 of 78.7 µg/mL, compared to ascorbic acid as a positive control with an IC50 of 10.6 µg/mL. It also demonstrated significant cytotoxic activity with IC50 values of 13.5 µg/mL and 25.3 µg/mL against HCT-116 and HEP-2 cell lines, respectively, compared to vinblastine as a positive control with IC50 values of 2.34 µg/mL and 6.61 µg/mL against HCT-116 and HEP-2, respectively. Additionally, the ethyl acetate fraction displayed promising antibacterial activity against S. aureus with a MIC value of 62.5 µg/mL, compared to ciprofloxacin as a positive control with MIC values of 1.56 µg/mL for Gram-positive bacteria and 3.125 µg/mL for Gram-negative bacteria. It also exhibited activity against E. coli and P. aeruginosa with MIC values of 250 µg/mL and 500 µg/mL, respectively. Briefly, this is the first report on the biological activities and secondary metabolite content of the ethyl acetate fraction of Hymedesmia sp. marine sponge, emphasizing the potential for further research against resistant bacterial and fungal strains, as well as different cancer cell lines. The ethyl acetate fraction of Hymedesmia sp. is a promising source of safe and unique natural drugs with potential therapeutic and pharmaceutical benefits.
摘要：
Hymedesmiidae是最大的海洋海绵家族之一，是具有多种生物活性的可变代谢物的特殊来源。在这项研究中,Hymedestmiasp.的乙酸乙酯级分（HE）。来自红海的海洋海绵，埃及,首次采用超高效液相色谱-电喷雾串联质谱(UPLC-ESI-MS/MS)分析。分析初步确定了该馏分中的29种化合物，包括六个化合物（两个嘧啶核苷，一个嘌呤,以及除一种脑苷脂外的两种嘧啶碱基）。通过1D和2DNMR（核磁共振）建立了分离化合物的结构，MS（质谱），和红外光谱。此外，细胞毒性，抗氧化剂，并在体外评估了乙酸乙酯级分的抗菌活性。该级分表现出强的DPPH清除活性，IC50为78.7µg/mL，与作为阳性对照的抗坏血酸相比，IC50为10.6µg/mL。它还表现出显著的细胞毒性活性，对HCT-116和HEP-2细胞系的IC50值为13.5μg/mL和25.3μg/mL，分别,与长春碱作为阳性对照相比，对HCT-116和HEP-2的IC50值分别为2.34µg/mL和6.61µg/mL。此外,乙酸乙酯部分对金黄色葡萄球菌显示出有希望的抗菌活性，MIC值为62.5µg/mL，与环丙沙星作为阳性对照相比，革兰氏阳性细菌的MIC值为1.56µg/mL，革兰氏阴性细菌的MIC值为3.125µg/mL。它还表现出对大肠杆菌和铜绿假单胞菌的活性，MIC值为250µg/mL和500µg/mL，分别。简而言之，这是有关Hymedesmiasp.的乙酸乙酯馏分的生物活性和次级代谢产物含量的第一份报告。海洋海绵,强调针对抗性细菌和真菌菌株的进一步研究的潜力，以及不同的癌细胞系。Hymedesmiasp.的乙酸乙酯级分。是具有潜在治疗和药物益处的安全和独特的天然药物的有希望的来源。
