PDF(Pubmed)
Abstract：
The psychedelic beverage ayahuasca is originally obtained by Banisteriopsis caapi (B. caapi) (BC) and Psychotria viridis (P. viridis) (PV). However, sometimes these plant species are replaced by others that mimic the original effects, such as Mimosa hostilis (M. hostilis) (MH) and Peganum harmala (P. harmala) (PH). Its worldwide consumption and the number of studies on its potential therapeutic effects has increased. This study aimed to evaluate the anticancer properties of ayahuasca in human colorectal adenocarcinoma cells. Thus, the maximum inhibitory concentration (IC50) of decoctions of MH, PH, and a mixture of these (MHPH) was determined. The activities of caspases 3 and 9 were evaluated, and the cell proliferation index was determined through immunocytochemical analysis (Ki-67). Two fluorescent probes were used to evaluate the production of oxidative stress and the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) was also evaluated. It was demonstrated that exposure to the extracts significantly induced apoptosis in Caco-2 cells, while decreasing cell proliferation. MH and MHPH samples significantly reduced oxidative stress and significantly increased glutathione peroxidase activity. No significant differences were found in SOD activity. Overall, it was demonstrated that the decoctions have a potential anticancer activity in Caco-2 cells.
摘要：
迷幻饮料ayahuasca最初是由Banisteriopsiscaapi（B.caapi)(BC)和病毒精神病(P.viridis)(PV)。然而,有时这些植物物种被其他模仿原始效果的植物物种所取代，如含羞草(M.hostilis)(MH)和Peganumharmala(P.harmala)(PH)。其全球消费量和关于其潜在治疗效果的研究数量有所增加。本研究旨在评估ayahuasca在人类结直肠腺癌细胞中的抗癌特性。因此，MH的最大抑制浓度（IC50），PH,并测定这些的混合物(MHPH)。对半胱天冬酶3和9的活性进行了评价，免疫细胞化学分析(Ki-67)测定细胞增殖指数。使用两种荧光探针来评估氧化应激的产生，并评估了抗氧化酶超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性。结果表明，暴露于提取物显著诱导Caco-2细胞凋亡，同时减少细胞增殖。MH和MHPH样品可显着降低氧化应激并显着增加谷胱甘肽过氧化物酶活性。SOD活性无显著差异。总的来说,结果表明，汤剂在Caco-2细胞中具有潜在的抗癌活性。
