PDF(Pubmed)
Abstract：
This study aimed to extract bioactive proteins and protein hydrolysates from Apis mellifera larvae and assess their potential application in cosmetics as well as their irritation properties. The larvae were defatted and extracted using various mediums, including DI water, along with 0.5 M aqueous solutions of sodium hydroxide, ascorbic acid, citric acid, and hydrochloric acid. Subsequently, the crude proteins were hydrolyzed using the Alcalase® enzyme. All extracts underwent testing for antioxidant activities via the 2,2\'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and Griess assays. Anti-aging properties were evaluated in terms of anti-collagenase and anti-hyaluronidase effects. Irritation potential was assessed using the hen\'s egg chorioallantoic membrane (HET-CAM) test. The results revealed that the sodium hydroxide extraction showed promising outcomes in terms of yield, protein content, and effectiveness in inhibiting hyaluronidase, with the highest inhibition at 78.1 ± 1.5%, comparable to that of oleanolic acid. Conversely, crude protein extracted with ascorbic acid and its hydrolysate showed notable antioxidant and collagenase-inhibitory activities. Remarkably, their anti-collagenase effects were comparable to those of ascorbic acid and lysine. Additionally, it demonstrated safety upon testing with the CAM. In conclusion, the findings provided valuable insights into the utilization of A. mellifera larval proteins as active ingredients with a wide range of cosmeceutical applications, particularly due to their antioxidant, anti-aging, and low irritation properties, which hold significant promise for anti-skin wrinkles.
摘要：
本研究旨在从Apismellifera幼虫中提取生物活性蛋白和蛋白水解产物，并评估其在化妆品中的潜在应用及其刺激特性。将幼虫脱脂并使用各种培养基提取，包括DI水,以及0.5M氢氧化钠水溶液，抗坏血酸,柠檬酸,还有盐酸.随后，使用Alcalase®酶水解粗蛋白。所有提取物均通过2,2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）和Griess测定法进行了抗氧化活性测试。在抗胶原酶和抗透明质酸酶作用方面评价抗衰老性质。使用鸡卵绒毛尿囊膜（HET-CAM）测试评估刺激潜力。结果表明，氢氧化钠萃取在产率方面显示出有希望的结果，蛋白质含量，和抑制透明质酸酶的有效性，最高抑制率为78.1±1.5%，与齐墩果酸相当。相反，用抗坏血酸及其水解物提取的粗蛋白具有明显的抗氧化和胶原酶抑制活性。值得注意的是,它们的抗胶原酶作用与抗坏血酸和赖氨酸相当.此外,它证明了安全与CAM测试。总之,这些发现为利用A.mellifera幼虫蛋白作为具有广泛药妆应用的活性成分提供了有价值的见解，特别是由于它们的抗氧化剂，抗衰老,和低刺激性，具有显著的抗皮肤皱纹的前景。
