PDF(Pubmed)
Abstract：
Cannabis contains numerous natural components and has several effects such as anticancer, anti-inflammatory and antioxidant. Cheungsam is a variety of non-drug-type hemp, developed in Korea and is used for fiber (stem) and oil (seed). The efficacy of Cheungsam on skin is not yet known, and although there are previous studies on Cheungsam seed oil, there are no studies on Cheungsam seed husk. In this study, we investigated the potential of Cheungsam seed husk ethanol extract (CSSH) to alleviate skin inflammation through evaluating the gene and protein expression levels of inflammatory mediators. The results showed that CSSH reduced pro-inflammatory cytokines (IL-1β, IL-6, IL-8, MCP-1 and CXCL10) and atopic dermatitis-related cytokines (IL-4, CCL17, MDC and RANTES) in TNF-α/IFN-γ-induced HaCaT cells. Furthermore, ERK, JNK and p38 phosphorylation were decreased and p-p65, p-IκBα, NLRP3, caspase-1, p-JAK1 and p-STAT6 were suppressed after CSSH treatment. CSSH significantly increased the level of the skin barrier factors filaggrin and involucrin. These results suggest that Cheungsam seed husk ethanol extract regulates the mechanism of skin inflammation and can be used as a new treatment for skin inflammatory diseases.
摘要：
大麻含有许多天然成分，具有抗癌等多种作用，抗炎和抗氧化剂。Cheungsam是多种非药物型大麻，在韩国开发，用于纤维（茎）和油（种子）。Cheungsam对皮肤的功效尚不清楚,尽管以前有关于昌沙籽油的研究，没有关于Cheungsam种子壳的研究。在这项研究中,我们通过评估炎症介质的基因和蛋白质表达水平，研究了Cheungsam籽壳乙醇提取物（CSSH）缓解皮肤炎症的潜力。结果表明，CSSH降低了促炎细胞因子(IL-1β，TNF-α/IFN-γ诱导的HaCaT细胞中的IL-6，IL-8，MCP-1和CXCL10）和特应性皮炎相关细胞因子（IL-4，CCL17，MDC和RANTES）。此外，ERK,JNK和p38磷酸化降低，p-p65，p-IκBα，CSSH处理后，NLRP3、caspase-1、p-JAK1和p-STAT6被抑制。CSSH显着增加了皮肤屏障因子聚丝团蛋白和总蛋白的水平。以上结果提示，川崎籽壳乙醇提取物具有调节皮肤炎症机制的作用，可作为一种治疗皮肤炎症疾病的新方法。
