PDF(Pubmed)
Abstract：
The present study was performed to investigate the negative impact of salinity on the growth of Chinese flowering cabbage (Brassica rapa ssp. chinensis var. parachinensis) and the ameliorative effects of quercetin dihydrate on the plant along with the elucidation of underlying mechanisms. The tolerable NaCl stress level was initially screened for the Chinese flowering cabbage plants during a preliminary pot trial by exposing the plants to salinity levels (0, 50, 100, 150, 200, 250, 300, 350, and 400 mM) and 250 mM was adopted for further experimentation based on the findings. The greenhouse experiment was performed by adopting a completely randomized design using three different doses of quercetin dihydrate (50, 100, 150 µM) applied as a foliar treatment. The findings showed that the exposure salinity significantly reduced shoot length (46.5%), root length (21.2%), and dry biomass (32.1%) of Chinese flowering cabbage plants. Whereas, quercetin dihydrate applied at concentrations of 100, and 150 µM significantly diminished the effect of salinity stress by increasing shoot length (36.8- and 71.3%), root length (36.57- and 56.19%), dry biomass production (51.4- and 78.6%), Chl a (69.8- and 95.7%), Chl b (35.2- and 87.2%), and carotenoid contents (21.4- and 40.3%), respectively, compared to the plants cultivated in salinized conditions. The data of physiological parameters showed a significant effect of quercetin dihydrate on the activities of peroxidase, superoxide dismutase, and catalase enzymes. Interestingly, quercetin dihydrate increased the production of medicinally important glucosinolate compounds in Chinese flowering cabbage plants. Molecular docking analysis showed a strong affinity of quercetin dihydrate with three different stress-related proteins of B. rapa plants. Based on the findings, it could be concluded that quercetin dihydrate can increase the growth of Chinese flowering cabbage under both salinity and normal conditions, along with an increase in the medicinal quality of the plants. Further investigations are recommended as future perspectives using other abiotic stresses to declare quercetin dihydrate as an effective remedy to rescue plant growth under prevailing stress conditions.
摘要：
进行本研究是为了研究盐度对大白菜生长的负面影响（Brassicarapassp。中国.parachinensis）以及槲皮素二水合物对植物的改善作用以及潜在机制的阐明。在初步的盆栽试验中，通过将植物暴露于盐度水平（0、50、100、150、200、250、300、350和400mM）和250mM，初步筛选了可容忍的NaCl胁迫水平。通过采用完全随机设计进行温室实验，使用三种不同剂量的槲皮素二水合物（50、100、150µM）作为叶面处理。结果表明，暴露盐度显着降低了芽长度（46.5％），根长（21.2%），和大白菜植物的干生物量（32.1％）。然而,槲皮素二水合物的浓度为100和150µM，通过增加枝长（36.8-和71.3％）显着减少了盐度胁迫的影响，根长（36.57-和56.19%），干生物质产量(51.4-和78.6%)，Chla（69.8-和95.7%），Chlb（35.2-和87.2%），和类胡萝卜素含量（21.4-和40.3%），分别,与盐碱化条件下种植的植物相比。生理参数数据表明，槲皮素二水对过氧化物酶活性有显著影响，超氧化物歧化酶,和过氧化氢酶。有趣的是,槲皮素二水合物增加了大白菜植物中药用重要的芥子油苷化合物的产生。分子对接分析表明，槲皮素二水合物与B.rapa植物的三种不同胁迫相关蛋白具有很强的亲和力。根据调查结果,可以得出结论，在盐度和正常条件下，槲皮素二水都可以增加大白菜的生长，随着植物药用质量的提高。建议使用其他非生物胁迫进行进一步的研究，以宣布槲皮素二水合物是在普遍胁迫条件下挽救植物生长的有效补救措施。
