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Abstract：
The increasing use of photoselective nets (PNs) raises the question of their influence on pollen traits. We aimed to evaluate the effect of PNs (yellow, pearl, and grey) on the pollen of \'Matua\' and \'Tomuri\' Actinidia deliciosa cultivars. The pollen size and the exine were studied with a light microscopy and a scanning electron microscopy, and the fertility was analysed by a viability assay and in vitro germination. The total soluble proteins (TSPs) and sugars (TSSs) were quantified by colorimetric assays. The molecular structure of the pollen grain\'s wall was analysed by a Raman spectroscopy. The pollen from the plants under the PNs had a larger width and area and a lower germination rate. No significant changes were observed in the exine\'s microperforations. The TSP and TSS contents were influenced by the cultivar and PNs (particularly the pearl PN). The Raman spectra of the pollen from the plants grown under the nets presented some bands that significantly shifted from their original position, indicating differences in the vibration modes of the molecules, but no overall changes at their structural or organisation level were found. Our study showed that the PNs could influence several pollen traits, with the pearl PN inducing greater modifications. Our results also support the idea that cultivars affect the outcome of some characteristics.
摘要：
光选择网（PNs）的使用日益增加，这引起了它们对花粉性状的影响。我们的目的是评估PN的效果(黄色，珍珠,和灰色）在\'Matua\'和\'Tomuri\'猕猴桃品种的花粉上。用光学显微镜和扫描电子显微镜研究了花粉大小和外壁，并通过生存力测定和体外萌发分析了生育力。通过比色测定对总可溶性蛋白(TSP)和糖(TSS)进行定量。通过拉曼光谱分析了花粉粒壁的分子结构。来自PNs下植物的花粉具有较大的宽度和面积以及较低的发芽率。在外壁的微穿孔中没有观察到明显的变化。TSP和TSS含量受品种和PN（尤其是珍珠PN）的影响。在网下生长的植物花粉的拉曼光谱显示出一些明显偏离其原始位置的条带，表明分子振动模式的差异，但没有发现结构或组织层面的整体变化。我们的研究表明，PNs可以影响几种花粉性状，与珍珠PN诱导更大的修改。我们的结果也支持以下观点：品种会影响某些特征的结果。
