PDF(Pubmed)
Abstract：
Cryopreservation is a promising method for the long-term preservation of plant germplasm, especially for vegetatively propagated species like freesias. In this study, we investigate streamlining the cryopreservation process for \'Sunny Gold\' Freesia, starting from effective in vitro initiation and proliferation using various plant growth regulator combinations. We also assess the impact of subculture on regrowth rates after cryopreservation. The shoot tips were successfully initiated in vitro after sterilization. The shoots were multiplied an average of three times in media containing N6-benzyladenine and kinetin. The regrowth rates of non-cryopreserved shoot tips excised from different subculture cycles did not differ significantly, with rates of 44% observed for plants from more than five subcultures and 47% for those from three subcultures. However, only the shoot tips excised from cultures subjected to three subculture cycles were able to recover after cryopreservation, with a regrowth rate of 31%. Our findings lay the groundwork for the development of an efficient cryopreservation protocol for freesias in the future.
摘要：
低温保存是一种很有前途的植物种质资源长期保存的方法,特别是对于像小菜鱼这样的营养繁殖的物种。在这项研究中,我们研究简化\“阳光黄金\”小苍兰的冷冻保存过程，从使用各种植物生长调节剂组合的有效体外启动和增殖开始。我们还评估了继代培养对冷冻保存后再生长率的影响。灭菌后，在体外成功启动了芽尖。在含有N6-苄基腺嘌呤和激动素的培养基中，将芽平均乘以三倍。从不同的继代培养周期中切除的非冷冻保存的茎尖的再生长率没有显着差异，来自五个以上传代培养的植物的比率为44％，来自三个传代培养的植物的比率为47％。然而,只有从经过三个继代培养周期的培养物中切除的茎尖能够在冷冻保存后恢复，再增长率为31%。我们的发现为将来开发有效的冷冻保存方案奠定了基础。
