PDF(Pubmed)
Abstract：
The discovery and investigation of new natural compounds with antimicrobial activity are new potential strategies to reduce the spread of antimicrobial resistance. The presented study reveals, for the first time, the promising antibacterial potential of two fractions from Cornu aspersum mucus with an MW < 20 kDa and an MW > 20 kDa against five bacterial pathogens-Bacillus cereus 1085, Propionibacterium acnes 1897, Salmonella enterica 8691, Enterococcus faecalis 3915, and Enterococcus faecium 8754. Using de novo sequencing, 16 novel peptides with potential antibacterial activity were identified in a fraction with an MW < 20 kDa. Some bioactive compounds in a mucus fraction with an MW > 20 kDa were determined via a proteomic analysis on 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and bioinformatics. High homology with proteins and glycoproteins was found, with potential antibacterial activity in mucus proteins named aspernin, hemocyanins, H-lectins, and L-amino acid oxidase-like protein, as well as mucins (mucin-5AC, mucin-5B, mucin-2, and mucin-17). We hypothesize that the synergy between the bioactive components determined in the composition of the fraction > 20 kDa are responsible for the high antibacterial activity against the tested pathogens in concentrations between 32 and 128 µg/mL, which is comparable to vancomycin, but without cytotoxic effects on model eukaryotic cells of Saccharomyces cerevisiae. Additionally, a positive effect, by reducing the levels of intracellular oxidative damage and increasing antioxidant capacity, on S. cerevisiae cells was found for both mucus extract fractions of C. aspersum. These findings may serve as a basis for further studies to develop a new antibacterial agent preventing the development of antibiotic resistance.
摘要：
具有抗菌活性的新天然化合物的发现和研究是减少抗菌素耐药性传播的新的潜在策略。这项研究显示，第一次,两种MW<20kDa且MW>20kDa的玉米花粘液级分对五种细菌病原体-蜡样芽孢杆菌1085，痤疮丙酸杆菌1897，肠沙门氏菌8691，粪肠球菌3915和屎肠球菌8754的潜在抗菌潜力。使用从头测序，在MW<20kDa的级分中鉴定了16种具有潜在抗菌活性的新肽。通过在12%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和生物信息学上的蛋白质组学分析确定MW>20kDa的粘液级分中的一些生物活性化合物。与蛋白质和糖蛋白有很高的同源性，在名为aspernin的粘液蛋白中具有潜在的抗菌活性，血蓝素,H-凝集素,和L-氨基酸氧化酶样蛋白，以及粘蛋白（粘蛋白-5AC，黏蛋白-5B,粘蛋白2和粘蛋白17）。我们假设，在>20kDa的组分中确定的生物活性成分之间的协同作用是针对浓度在32至128µg/mL之间的测试病原体的高抗菌活性的原因。与万古霉素相当，但对酿酒酵母模型真核细胞没有细胞毒性作用。此外,积极的影响，通过降低细胞内氧化损伤水平和增加抗氧化能力，在酿酒酵母细胞上发现了苹果的两种粘液提取物级分。这些发现可以作为进一步研究的基础，以开发一种新的抗菌剂来防止抗生素耐药性的发展。
