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Abstract：
In this study, we report the successful development of a novel high-sensitivity intensity-based Surface Plasmon Resonance imaging (SPRi) biosensor and its application for detecting molecular interactions. By optimizing the excitation wavelength and employing a wavelength division multiplexing (WDM) algorithm, the system can determine the optimal excitation wavelength based on the initial refractive index of the sample without adjusting the incidence angle. The experimental results demonstrate that the refractive index resolution of the system reaches 1.77×10-6 RIU. Moreover, it can obtain the optimal excitation wavelength for samples with an initial refractive index in the range of 1.333 to 1.370 RIU and accurately monitor variations within the range of 0.0037 RIU without adjusting the incidence angle. Additionally, our new SPRi technique realized real-time detection of high-throughput biomolecular binding processes, enabling analysis of kinetic parameters. This research is expected to advance the development of more accurate SPRi technologies for molecular interaction analysis.
摘要：
在这项研究中,我们报道了一种新型高灵敏度基于强度的表面等离子体共振成像（SPRi）生物传感器的成功开发及其在检测分子相互作用中的应用。通过优化激发波长并采用波分复用（WDM）算法，该系统可以根据样品的初始折射率确定最佳激发波长，而无需调整入射角。实验结果表明，该系统的折射率分辨率达到1.77×10-6RIU。此外，它可以获得初始折射率在1.333至1.370RIU范围内的样品的最佳激发波长，并精确监测0.0037RIU范围内的变化，而无需调整入射角。此外,我们的新SPRi技术实现了高通量生物分子结合过程的实时检测，能够分析动力学参数。这项研究有望促进更精确的SPRi分子相互作用分析技术的发展。
