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Abstract：
A tumor-targeting fluorescent probe has attracted increasing interest in fluorescent imaging for the noninvasive detection of cancers in recent years. Sulfonamide-containing naphthalimide derivatives (SN-2NI, SD-NI) were synthesized by the incorporation of N-butyl-4-ethyldiamino-1,8-naphthalene imide (NI) into sulfonamide (SN) and sulfadiazine (SD) as the tumor-targeting groups, respectively. These derivatives were further characterized by mass spectrometry (MS), nuclear magnetic resonance spectroscopy (1H NMR), Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV), and a fluorescence assay. In vitro properties, including cell cytotoxicity and the cell uptake of tumor cells, were also evaluated. Sulfonamide-containing naphthalimide derivatives possessed low cell cytotoxicity to B16F10 melanoma cells. Moreover, SN-2NI and SD-NI can be taken up highly by B16F10 cells and then achieve good green fluorescent images in B16F10 cells. Therefore, sulfonamide-containing naphthalimide derivatives can be considered to be the potential probes used to target fluorescent imaging in tumors.
摘要：
近年来，靶向肿瘤的荧光探针在用于癌症的非侵入性检测的荧光成像中引起了越来越多的兴趣。含磺酰胺的萘二甲酰亚胺衍生物（SN-2NI，SD-NI）是通过将N-丁基-4-乙基二氨基-1,8-萘酰亚胺（NI）掺入磺酰胺（SN）和磺胺嘧啶（SD）作为肿瘤靶向基团来合成的，分别。这些衍生物通过质谱(MS)进一步表征,核磁共振波谱(1HNMR),傅里叶变换红外光谱(FT-IR),紫外-可见光谱(UV),和荧光分析。体外特性，包括细胞毒性和肿瘤细胞的细胞摄取，也进行了评估。含磺酰胺的萘二甲酰亚胺衍生物对B16F10黑色素瘤细胞具有低细胞毒性。此外，SN-2NI和SD-NI可以被B16F10细胞高度摄取，然后在B16F10细胞中获得良好的绿色荧光图像。因此,含磺酰胺的萘酰亚胺衍生物可以被认为是用于在肿瘤中靶向荧光成像的潜在探针。
