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Abstract：
An RP-HPLC method with a UV detector was developed for the simultaneous quantification of diclofenac diethylamine, methyl salicylate, and capsaicin in a pharmaceutical formulation and rabbit skin samples. The separation was achieved using a Thermo Scientific ACCLAIMTM 120 C18 column (Waltham, MA, USA, 4.6 mm × 150 mm, 5 µm). The optimized elution phase consisted of deionized water adjusted to pH = 3 using phosphoric acid mixed with acetonitrile in a 35:65% (v/v) ratio with isocratic elution. The flow rate was set at 0.7 mL/min, and the detection was performed at 205 nm and 25 °C. The method exhibits good linearity for capsaicin (0.05-70.0 µg/mL), methyl salicylate (0.05-100.0 µg/mL), and diclofenac diethylamine (0.05-100.0 µg/mL), with low LOD values (0.0249, 0.0271, and 0.0038 for capsaicin, methyl salicylate, and diclofenac diethylamine, respectively). The RSD% values were below 3.0%, indicating good precision. The overall greenness score of the method was 0.61, reflecting its environmentally friendly nature. The developed RP-HPLC method was successfully applied to analyze Omni Hot Gel® pharmaceutical formulation and rabbit skin permeation samples.
摘要：
建立了具有UV检测器的RP-HPLC方法，用于同时定量双氯芬酸二乙胺。水杨酸甲酯,和辣椒素在药物制剂和兔皮肤样品中。分离是使用ThermoScientificACCLAIMTM120C18色谱柱(Waltham，MA,美国,4.6mm×150mm,5µm)。优化的洗脱相由去离子水组成,所述去离子水使用磷酸与乙腈以35:65%(v/v)的比例混合调节至pH=3,具有等度洗脱。流速设定为0.7mL/min,并且在205nm和25°C下进行检测。该方法对辣椒素（0.05-70.0µg/mL）具有良好的线性，水杨酸甲酯（0.05-100.0µg/mL），和双氯芬酸二乙胺（0.05-100.0µg/mL），低LOD值（辣椒素为0.0249、0.0271和0.0038，水杨酸甲酯,和双氯芬酸二乙胺,分别)。RSD%值低于3.0%，显示良好的精度。该方法的总体绿色评分为0.61，反映了其环境友好性。所开发的RP-HPLC方法成功地应用于分析OmniHotGel®药物制剂和兔皮肤渗透样品。
