PDF(Pubmed)
Abstract：
The detection of the amount of aflatoxin M1 (AFM1) in milk is crucial for food safety. Here, we utilize a fiber optic (FO) localized surface plasmon resonance (LSPR) biosensor by constructing gold nanoparticle (AuNP) multimers, in which the nanogaps amplified the LSPR signal by the hot spot effect, and achieved a highly sensitive detection of f AFM1. Through the optimization of parameter conditions for the fabrication of the sensor and detection system, a high performance result from the FO LSPR biosensor was obtained, and the method for AFM1 detection was established, with a wide detection range of 0.05-100 ng/mL and a low limit of detection (LOD) of 0.04 ng/mL, and it has been successfully validated with the actual sample milk. Therefore, it is a good strategy to fabricate highly sensitive FO LSPR sensors for detecting AFM1 by constructing AuNP multimers, and this approach is suitable for developing other biosensors.
摘要：
牛奶中黄曲霉毒素M1(AFM1)的检测对食品安全至关重要。这里,我们利用光纤（FO）局域表面等离子体共振（LSPR）生物传感器通过构建金纳米粒子（AuNP）多聚体，其中纳米间隙通过热点效应放大了LSPR信号，并实现了对fAFM1的高灵敏度检测。通过对传感器和检测系统制作参数条件的优化,从FOLSPR生物传感器获得了高性能的结果，建立了AFM1检测方法,具有0.05-100ng/mL的宽检测范围和0.04ng/mL的低检测限（LOD），它已经成功地验证了实际样品牛奶。因此,通过构建AuNP多聚体来制造高灵敏度的FOLSPR传感器来检测AFM1是一个很好的策略，这种方法适用于开发其他生物传感器。
