PDF(Pubmed)
Abstract：
Antibiotics are routinely added to ornamental fish tanks for treating bacterial infection or as a prophylactic measure. However, the overuse or subtherapeutical application of antibiotics could potentially facilitate the selection of antibiotic resistance in bacteria, yet no studies have investigated antibiotic use in the retail ornamental fish sector and its impact on microbial communities. The present study analyzed the concentrations of twenty antibiotics in the carriage water (which also originates from fish tanks in retail shops) collected monthly from ten local ornamental fish shops over a duration of three months. The antibiotic concentrations were correlated with the sequenced microbial community composition, and the risk of resistance selection in bacteria was assessed. Results revealed that the detected concentrations of tetracyclines were the highest among samples, followed by fluoroquinolones and macrolides. The concentrations of oxytetracycline (44.3 to 2,262,064.2 ng L-1) detected across three months demonstrated a high risk for resistance selection at most of the sampled shops. Zoonotic pathogens (species of Rhodococcus, Legionella, and Citrobacter) were positively correlated with the concentrations of oxytetracycline, tetracycline, chlortetracycline, and enrofloxacin. This suggests that antibiotic use in retail shops may increase the likelihood of selecting for zoonotic pathogens. These findings shed light on the potential for ornamental fish retail shops to create a favorable environment for the selection of pathogens with antibiotics, thereby highlighting the urgent need for enhanced antibiotic stewardship within the industry.
摘要：
抗生素通常被添加到观赏性鱼缸中用于治疗细菌感染或作为预防措施。然而,抗生素的过度使用或亚治疗应用可能潜在地促进细菌中抗生素耐药性的选择，然而，没有研究调查抗生素在零售观赏鱼行业的使用及其对微生物群落的影响。本研究分析了在三个月的时间内每月从10家当地观赏鱼商店收集的运输水中20种抗生素的浓度（也来自零售店的鱼缸）。抗生素浓度与测序的微生物群落组成相关，并评估细菌耐药性选择的风险。结果显示，四环素的检测浓度在样品中最高，其次是氟喹诺酮类和大环内酯类。在三个月内检测到的土霉素浓度（44.3至2,262,064.2ngL-1）表明，大多数采样商店的耐药性选择风险很高。人畜共患病病原体（红球菌的种类，军团菌,和柠檬酸杆菌）与土霉素的浓度呈正相关，四环素,金霉素,和恩诺沙星.这表明在零售店使用抗生素可能会增加选择人畜共患病原体的可能性。这些发现揭示了观赏鱼零售店为抗生素病原体的选择创造有利环境的潜力，从而凸显了行业内加强抗生素管理的迫切需要。
