PDF(Pubmed)
Abstract：
Bacterial diversity and its distribution characteristics in sediments are critical to understanding and revealing biogeochemical cycles in sediments. However, little is known about the relationship between biogeochemistry processes and vertical spatial distribution of bacterial communities in sandy sediments. In this study, we used fluorescence quantitative PCR, high-throughput sequencing technology and statistical analysis to explore the vertical distribution pattern of bacterial community diversity and its influencing factors in sandy sediments of the Yangtze River Basin. The aim is to enrich the understanding of the ecological characteristics and functions of bacteria in river ecosystems. The results showed that both sediment bacterial abundance and diversity showed a gradual decrease from surface to bottom in the vertical distribution. The main environmental factors that influenced the bacterial distribution pattern were pore water dissolved oxygen (DO), total nitrogen (TN) concentration and sediment nitrogen (N) content. The dominant bacterial species, Massilia and Flavobacterium, are suitable for growth and reproduction in high oxygen and nutrient-richer environments, while Limnobacter prefers low oxygen or anaerobic conditions. The vertical distribution pattern of bacteria and its influencing factors in river sandy sediment found in this study differ from the results in mud sediment, which may be related to the larger granular gap between sandy sediment and the lower content of organic matter. The findings of this study further our understanding of the distribution patterns and ecological preferences of microbial communities in river sediments, providing insights into how these communities may adapt to varying environmental conditions.
摘要：
沉积物中细菌多样性及其分布特征对于理解和揭示沉积物中的生物地球化学循环至关重要。然而,关于沙质沉积物中生物地球化学过程与细菌群落的垂直空间分布之间的关系知之甚少。在这项研究中,我们用荧光定量PCR,高通量测序技术和统计分析,探讨长江流域沙质沉积物细菌群落多样性的垂直分布格局及其影响因素。旨在丰富对河流生态系统中细菌的生态特征和功能的认识。结果表明，沉积物细菌丰度和多样性在垂直分布上从表面到底部呈逐渐降低的趋势。影响细菌分布规律的主要环境因素是孔隙水溶解氧(DO),总氮(TN)浓度和沉积物氮(N)含量。优势细菌种类,Massilia和黄杆菌属，适合在高氧气和营养丰富的环境中生长和繁殖，而Limnobacter更喜欢低氧或厌氧条件。本研究发现河流沙质沉积物中细菌的垂直分布格局及其影响因素与泥质沉积物中的结果不同,这可能与沙质沉积物之间较大的颗粒间隙和较低的有机质含量有关。这项研究的结果进一步了解了河流沉积物中微生物群落的分布模式和生态偏好，提供这些社区如何适应不同的环境条件的见解。
