PDF(Pubmed)
Abstract：
A Gram-positive, rod-shaped, aerobic, motile, and spore-forming bacterium, designated SCL10, was isolated from Acaudina molpadioides exposure to Co-60 radiation. In this study, whole-genome sequencing was performed to identify the strain as Bacillus cereus and functional characterization, with a focus on stress resistance. The genome of the B. cereus SCL10 strain was sequenced and assembled, revealing a size of 4,979,182 bp and 5167 coding genes. The genes involved in biological functions were annotated by using the GO, COG, KEGG, NR, and Swiss-Prot databases. The results showed that genes related to alkyl hydroperoxide reductase (ahpC, ahpF), DNA-binding proteins from starved cells (dps), spore and biofilm formation (spoVG, spo0A, gerP), cold shock-like protein (cspC, cspE), ATP-dependent chaperone (clpB), and photolyase, small, acid-soluble spore protein (SASP) and DNA repair protein (recA, radD) could explain the stress resistance. These findings suggest that antioxidant activity, sporulation, biofilm formation, and DNA protection may be considered as the main resistance mechanisms under exposure to radiation in the B. cereus SCL10 strain.
摘要：
革兰氏阳性，杆状,有氧,能动,和孢子形成细菌，命名为SCL10，是从暴露于Co-60辐射的Acaudinamolpadioides中分离出来的。在这项研究中,进行了全基因组测序，以鉴定菌株为蜡样芽孢杆菌和功能表征，专注于抗压力。对蜡状芽孢杆菌SCL10菌株的基因组进行测序和组装，揭示了4,979,182bp的大小和5167个编码基因。通过使用GO注释参与生物学功能的基因，COG,KEGG,NR,和Swiss-Prot数据库。结果表明，与烷基过氧化氢还原酶（ahpC，ahpF),来自饥饿细胞的DNA结合蛋白（dps），孢子和生物膜的形成(spoVG，spo0A,GerP),冷休克样蛋白(cspC，cspE),ATP依赖性伴侣（clpB），和光解酶，小,酸溶性孢子蛋白(SASP)和DNA修复蛋白(recA，radD)可以解释抗应力性。这些发现表明，抗氧化活性，孢子形成,生物膜的形成，和DNA保护可能被认为是蜡状芽孢杆菌SCL10菌株在暴露于辐射下的主要抗性机制。
