PDF(Pubmed)
Abstract：
Soil bacterial communities play a remarkable role in nutrient cycling, significantly affecting soil organic material content, soil fertility, and, in an indirect way, plant succession processes. Conversely, vegetation type influences microbial soil life. The present study compared the bacterial microbiome composition, diversity and catabolic activity profile of topsoil samples collected under three different forest types (a twice-coppiced black locust stand, a young, naturally reforested, and a middle-aged mixed pedunculate oak stand) planted on former arable land in the early 20th century. Diversity indices determined during 16S ribosomal RNA sequencing-based metagenome analysis indicated that the black locust stand had the highest soil bacterial community diversity. At the phylum level, Acidobacteriota, Actinobacteriota, Proteobacteria, Verrucomicrobiota, Bacteroidota, and Gemmatimonadota were the most abundant taxa in the forest soils. Concerning soil parameters, redundancy analysis revealed that pH had the highest impact on bacterial community structure and pH, and soil organic carbon content on the samples\' respiration patterns. As for catabolic activity, the recently clearcut oak forest showed the lowest substrate-induced respiration, and citrate was the main driver for the inter-stand variability of microbial activity. Our results confirm that soil parameters and forest type influence the composition and functioning of the soil bacterial microbiome.
摘要：
土壤细菌群落在养分循环中起着显著的作用,显著影响土壤有机质含量，土壤肥力,and,以间接的方式，植物演替过程。相反，植被类型影响微生物土壤生命。本研究比较了细菌微生物组组成，在三种不同森林类型下收集的表层土壤样品的多样性和分解代谢活动概况（两次复制的刺槐林，一个年轻人,自然重新造林，和20世纪初在以前的耕地上种植的中年混合花梗橡树林）。在基于16S核糖体RNA测序的宏基因组分析中确定的多样性指数表明，刺槐林具有最高的土壤细菌群落多样性。在门一级，酸杆菌,放线菌,变形杆菌,Verrucomicrobiota,拟杆菌,和Gemmaatimonadota是森林土壤中最丰富的类群。关于土壤参数，冗余分析表明，pH对细菌群落结构和pH的影响最大，和样品呼吸模式上的土壤有机碳含量。至于分解代谢活动，最近砍伐的橡树林显示出最低的基质诱导呼吸，柠檬酸盐是微生物活性差异的主要驱动因素。我们的结果证实，土壤参数和森林类型会影响土壤细菌微生物组的组成和功能。
