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Abstract：
This study extensively analyzed the bacterial information of biofilms and activated sludge in oxic reactors of full-scale moving bed biofilm reactor-integrated fixed-film activated sludge (MBBR-IFAS) systems. The bacterial communities of biofilms and activated sludge differed statistically (R = 0.624, p < 0.01). The denitrifying genera Ignavibacterium, Phaeodactylibacter, Terrimonas, and Arcobacter were more abundant in activated sludge (p < 0.05), while comammox Nitrospira was more abundant in biofilms (p < 0.05), with an average relative abundance of 8.13%. Nitrospira and Nitrosomonas had weak co-occurrence relationships with other genera in the MBBR-IFAS systems. Potential function analysis revealed no differences in pathways at levels 1 and 2 based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) between biofilms and activated sludge. However, in terms of pathways at level 3, biofilms had more potential in 26 pathways, including various organic biodegradation and membrane and signal transportation pathways. In comparison, activated sludge had more potential in only five pathways, including glycan biosynthesis and metabolism. With respect to nitrogen metabolism, biofilms had greater potential for nitrification (ammonia oxidation) (M00528), and complete nitrification (comammox) (M00804) concretely accounted for methane/ammonia monooxygenase (K10944, K10945, and K10946) and hydroxylamine dehydrogenase (K10535). This study provides a theoretical basis for MBBR-IFAS systems from the perspective of microorganisms.
摘要：
这项研究广泛分析了全尺寸移动床生物膜反应器-集成固定膜活性污泥（MBBR-IFAS）系统的含氧反应器中生物膜和活性污泥的细菌信息。生物膜和活性污泥的细菌群落差异有统计学意义（R=0.624，p<0.01）。反硝化属Ignavibacterium,Phaeodactylibacter,Terrimonas,活性污泥中的杆菌含量更高(p<0.05)，而在生物膜中，硝基螺旋体中的Comammox更丰富（p<0.05），平均相对丰度为8.13%。在MBBR-IFAS系统中，硝基螺旋体和硝基螺旋体与其他属的共生关系较弱。潜在功能分析显示，基于京都基因和基因组百科全书（KEGG），生物膜和活性污泥之间的1级和2级途径没有差异。然而,就第3级的途径而言，生物膜在26个途径中具有更大的潜力，包括各种有机生物降解和膜和信号传输途径。相比之下,活性污泥仅在五种途径中具有更大的潜力，包括聚糖的生物合成和代谢。关于氮代谢，生物膜具有更大的硝化（氨氧化）潜力（M00528），和完全硝化(Comammox)(M00804)具体考虑甲烷/氨单加氧酶(K10944、K10945和K10946)和羟胺脱氢酶(K10535)。本研究从微生物的角度为MBBR-IFAS系统提供了理论依据。
