PDF(Pubmed)
Abstract：
Multidrug-resistant (MDR) Escherichia coli poses a significant threat to public health, contributing to elevated rates of morbidity, mortality, and economic burden. This study focused on investigating the antibiotic resistance profiles, resistance and virulence gene distributions, biofilm formation capabilities, and sequence types of E. coli strains resistant to six or more antibiotic classes. Among 918 strains isolated from 33 wastewater treatment plants (WWTPs), 53.6% (492/918) demonstrated resistance, 32.5% (298/918) were MDR, and over 8% (74/918) were resistant to six or more antibiotic classes, exhibiting complete resistance to ampicillin and over 90% to sulfisoxazole, nalidixic acid, and tetracycline. Key resistance genes identified included sul2, blaTEM, tetA, strA, strB, and fimH as the predominant virulence genes linked to cell adhesion but limited biofilm formation; 69% showed no biofilm formation, and approximately 3% were strong producers. Antibiotic residue analysis detected ciprofloxacin, sulfamethoxazole, and trimethoprim in all 33 WWTPs. Multilocus sequence typing analysis identified 29 genotypes, predominantly ST131, ST1193, ST38, and ST69, as high-risk clones of extraintestinal pathogenic E. coli. This study provided a comprehensive analysis of antibiotic resistance in MDR E. coli isolated from WWTPs, emphasizing the need for ongoing surveillance and research to effectively manage antibiotic resistance.
摘要：
多药耐药（MDR）大肠杆菌对公众健康构成重大威胁，导致发病率上升，死亡率,和经济负担。这项研究的重点是调查抗生素的耐药性，抗性和毒力基因分布，生物膜形成能力，和对六种或更多抗生素类具有抗性的大肠杆菌菌株的序列类型。在从33个污水处理厂（WWTP）中分离出的918个菌株中，53.6%(492/918)表现出抗性，32.5%(298/918)是MDR，超过8％（74/918）对六种或更多抗生素具有抗性，表现出对氨苄青霉素的完全耐药性，对磺胺异恶唑的耐药性超过90%，萘啶酸,还有四环素.确定的关键抗性基因包括sul2，blaTEM，tetA,strA,strB,和fimH作为与细胞粘附相关的主要毒力基因，但限制了生物膜的形成；69％显示没有生物膜形成，大约3%是强大的生产者。抗生素残留分析检测到环丙沙星，磺胺甲恶唑,和甲氧苄啶在所有33个WWTP中。多位点序列分型分析确定了29种基因型，主要是ST131，ST1193，ST38和ST69，作为肠外致病性大肠杆菌的高风险克隆。这项研究提供了从污水处理厂分离的MDR大肠杆菌的抗生素耐药性的综合分析，强调需要持续的监测和研究，以有效管理抗生素耐药性。
