PDF(Pubmed)
Abstract：
(1) Background: Antibiotic resistance in bacteria is an urgent global threat to public health. Migratory birds can acquire antibiotic-resistant and pathogenic bacteria from the environment or through contact with each other and spread them over long distances. The objectives of this study were to explore the relationship between migratory birds and the transmission of drug-resistant pathogenic Escherichia coli. (2) Methods: Faeces and swab samples from migratory birds were collected for isolating E. coli on the Inner Mongolia Plateau of northern China from 2018 to 2023. The resistant phenotypes and spectra of isolates were determined using a BD Phoenix 100 System. Conjugation assays were performed on extended-spectrum β-lactamase (ESBL)-producing strains, and the genomes of multidrug-resistant (MDR) and ESBL-producing isolates were sequenced and analysed. (3) Results: Overall, 179 isolates were antibiotic-resistant, with 49.7% MDR and 14.0% ESBL. Plasmids were successfully transferred from 32% of ESBL-producing strains. Genome sequencing analysis of 91 MDR E. coli strains identified 57 acquired resistance genes of 13 classes, and extraintestinal pathogenic E. coli and avian pathogenic E. coli accounted for 26.4% and 9.9%, respectively. There were 52 serotypes and 54 sequence types (STs), including ST48 (4.4%), ST69 (4.4%), ST131 (2.2%) and ST10 (2.2%). The international high-risk clonal strains ST131 and ST10 primarily carried blaCTX-M-27 and blaTEM-176. (4) Conclusions: There is a high prevalence of multidrug-resistant virulent E. coli in migratory birds on the Inner Mongolian Plateau. This indicates a risk of intercontinental transmission from migratory birds to livestock and humans.
摘要：
(1)研究背景：细菌中的抗生素耐药性是对公众健康的一个紧迫的全球性威胁。候鸟可以从环境中或通过相互接触获得抗生素抗性和致病性细菌，并将它们传播到很远的距离。本研究的目的是探讨候鸟与耐药致病性大肠杆菌传播的关系。(2)方法:2018-2023年采集候鸟粪便和拭子样本,在中国北方内蒙古高原分离大肠杆菌。使用BDPhoenix100系统确定分离株的抗性表型和光谱。在产超广谱β-内酰胺酶（ESBL）的菌株上进行缀合测定，并对产生多药耐药（MDR）和ESBL的分离株的基因组进行了测序和分析。（3）结果：总体上，179株对抗生素耐药，具有49.7%的MDR和14.0%的ESBL。成功地从32%的产ESBL菌株中转移了质粒。91株MDR大肠杆菌的基因组测序分析确定了13类的57个获得性抗性基因，肠外致病性大肠杆菌和禽致病性大肠杆菌分别占26.4%和9.9%，分别。有52个血清型和54个序列类型（STs），包括ST48（4.4%），ST69（4.4%），ST131（2.2%）和ST10（2.2%）。国际高风险克隆菌株ST131和ST10主要携带blaCTX-M-27和blaTEM-176。(4)结论:内蒙古高原候鸟体内多重耐药强大肠杆菌的流行率较高。这表明从候鸟到牲畜和人类的洲际传播的风险。
