PDF(Pubmed)
Abstract：
Parvovirus infection affects several animal species, especially young animals. In birds, parvovirus infection has been described in Muscovy ducks, turkeys, and chickens, all of which had enteric diseases characterized by diarrhea. Chicken parvovirus (ChPV) has been detected in poultry around the world in animals affected by enteric problems, showing dwarfism, cloacal pasting, and diarrhea. In Brazil, ChPV was detected in chickens affected by diarrhea fifteen years ago. However, the genetic characteristics of ChPV circulating in chicken flocks were not determined. Therefore, the aim of the present investigation was to determine the genetic characteristics of the VP1 gene from ChPV detected in chickens affected by enteric diseases in Brazil. For this purpose, a molecular approach was used. Specific primers were designed to flank the complete VP1 gene of ChPV and amplify it using PCR. The amplified products from samples of chickens with enteric diseases were sequenced, and 22 complete CDs of the VP1 gene were obtained. These samples, compared to the ABU-P1 sequence, showed 17 sequences with high nucleotide (NT) similarity of 92.7-97.4% and amino acid (AA) similarity of 94.8-99.5% associated with Runting and Stunting syndrome (RSS); there were also five samples associated with hens with diarrhea with unusual jejunal dilatation (JD) that had less similarity than the RSS sequences (NT of 86.5% and AA of 93-93.1%). The phylogenetic analysis determined four groups. Group I had sequences from Korea. The second group included sequences from Korea, China, and Brazil (not included in this work). The third group had studied RSS sequences grouped with the ABU-P1 strain and sequences from China and the United States. Finally, the sequences from JD were clustered in a separate group with a bootstrap of 100%, a group that was denoted as group IV, and included sequences from China. RDP4 and SimPlot analysis showed one point of recombination with the sequences of group III ChPV in the JD sequences. Herein, we show that circulating strains of ChPV exhibit genetic differences in the VP1 gene in Brazilian chicken flocks. Nevertheless, more studies are needed to determine the probability of a new genetic group of ChPV based on the analysis of the complete genome.
摘要：
细小病毒感染会影响几种动物，尤其是年轻的动物。在鸟类中，已经在番鸭中描述了细小病毒感染，火鸡,和鸡,所有这些都有以腹泻为特征的肠道疾病。鸡细小病毒（ChPV）已在世界各地的家禽中检测到受肠道疾病影响的动物，显示侏儒症，泄殖腔粘贴，和腹泻。在巴西,15年前在受腹泻影响的鸡中检测到ChPV。然而,尚未确定在鸡群中循环的ChPV的遗传特征。因此,本研究的目的是确定在巴西受肠道疾病影响的鸡中检测到的ChPV的VP1基因的遗传特征。为此,使用了分子方法。设计特异性引物以侧接ChPV的完整VP1基因并使用PCR对其进行扩增。对患有肠道疾病的鸡样本的扩增产物进行了测序，并获得了22个完整的VP1基因CD。这些样本,与ABU-P1序列相比，显示17个序列的高核苷酸（NT）相似性为92.7-97.4％，氨基酸（AA）相似性为94.8-99.5％，与长跑和发育迟缓综合征（RSS）相关；还有五个与腹泻的母鸡具有异常空肠扩张（JD）的相似度低于RSS序列（NT为86.5％，AA为93-93.1％）。系统发育分析确定了四个组。第一组有来自韩国的序列。第二组包括来自韩国的序列，中国,和巴西（不包括在这项工作中）。第三组研究了与ABU-P1株以及来自中国和美国的序列分组的RSS序列。最后,来自JD的序列聚集在一个单独的组中，具有100%的引导，一组被称为第四组，包括来自中国的序列。RDP4和SimPlot分析显示了与JD序列中III组ChPV序列的一个重组点。在这里，我们表明，ChPV的循环菌株在巴西鸡群中表现出VP1基因的遗传差异。然而，需要更多的研究来确定一个新的ChPV遗传群体的概率基于完整的基因组分析。
