PDF(Pubmed)
Abstract：
Rapid industrial growth is associated with an increase in the production of environmentally harmful waste. A potential solution to significantly reduce pollution is to replace current synthetic materials with readily biodegradable plastics. Moreover, to meet the demands of technological advancements, it is essential to develop materials with unprecedented properties to enhance their functionality. Polysaccharide composites demonstrate significant potential in this regard. Polysaccharides possess exceptional film-forming abilities and are safe for human use, biodegradable, widely available, and easily modifiable. Unfortunately, polysaccharide-based films fall short of meeting all expectations. To address this issue, the current study focused on incorporating carbon quantum dots (CQDs), which are approximately 10 nm in size, into the structure of a starch/chitosan biocomposite at varying concentrations. This modification has improved the mechanical properties of the resulting nanocomposites. The inclusion of nanoparticles led to a slight reduction in solubility and an increase in the swelling degree. The optical characteristics of the obtained films were influenced by the presence of CQDs, and the fluorescence intensity of the nanocomposites changed due to the specific heavy metal ions and amino acids used. Consequently, these nanocomposites show great potential for detecting these compounds. Cellular viability assessments and comet assays confirm that the resulting nanocomposites do not exhibit any cytotoxic properties based on this specific analytic method. The tested nanocomposites with the addition of carbon quantum dots (NC/CD II and NC/CD III) were characterised by greater genotoxicity compared to the negative control. The positive control, the starch/chitosan composite alone, was also characterised by a greater induction of chromatin damage in mouse cells compared to a pure mouse blood sample.
摘要：
快速的工业增长与对环境有害的废物产生的增加有关。显着减少污染的潜在解决方案是用易于生物降解的塑料代替当前的合成材料。此外，为了满足技术进步的要求，开发具有前所未有性能的材料以增强其功能至关重要。多糖复合物在这方面显示出显著的潜力。多糖具有特殊的成膜能力，对人类使用是安全的，可生物降解，广泛可用，并且易于修改。不幸的是,基于多糖的薄膜未能满足所有期望。为了解决这个问题，目前的研究集中在掺入碳量子点(CQDs)，尺寸约为10纳米，不同浓度的淀粉/壳聚糖生物复合材料的结构。这种改性改善了所得纳米复合材料的机械性能。纳米颗粒的包含导致溶解度的轻微降低和溶胀度的增加。获得的薄膜的光学特性受到CQDs存在的影响，纳米复合材料的荧光强度由于所使用的特定重金属离子和氨基酸而改变。因此,这些纳米复合材料显示出检测这些化合物的巨大潜力。细胞活力评估和彗星测定证实,基于该特定分析方法,所得纳米复合材料不表现出任何细胞毒性性质。与阴性对照相比，添加碳量子点的测试纳米复合材料（NC/CDII和NC/CDIII）的特征在于更大的遗传毒性。阳性对照，单独的淀粉/壳聚糖复合材料，与纯小鼠血液样品相比，小鼠细胞中染色质损伤的诱导作用更大。
