PDF(Pubmed)
Abstract：
Prolamins, proteins derived from plants, have extensive applications in pharmaceutics and food science. Jiuzao is a byproduct of the Baijiu brewing industry, and is a great source of prolamin. Despite its importance, knowledge regarding the extraction techniques and the properties of prolamin derived from Baijiu Jiuzao (PBJ) remains limited. Reverse micelles (RMs) extraction offers an efficient and cost-effective method for purifying proteins. In the present study, prolamin was extracted from Baijiu Jiuzao using RMs extraction and subsequently characterized in terms of its secondary structure, morphology, and particle size distribution. Our findings indicate that the purified prolamin extracted using further RMs extraction possessed higher α-helix content (+13.25%), forming a large-scale protein network, and narrower particle size distributions compared to the crude prolamin obtained by NaOH-ethanol method. This research suggests that RMs extraction has potential applications in extracting prolamin from brewing industry byproducts, offering an environmentally friendly approach to Baijiu Jiuzao recycling.
摘要：
醇溶蛋白,来自植物的蛋白质，在制药和食品科学中有广泛的应用。九灶是白酒酿造工业的副产品，是醇溶蛋白的重要来源。尽管它很重要,关于白酒九灶(PBJ)醇溶蛋白提取技术和性质的知识仍然有限。反胶束（RM）提取为纯化蛋白质提供了一种有效且具有成本效益的方法。在本研究中,醇溶蛋白是使用RM提取方法从白酒九灶中提取的，随后对其二级结构进行了表征，形态学,和粒度分布。我们的发现表明，使用进一步的RM提取提取的纯化的醇溶蛋白具有更高的α-螺旋含量（13.25％），形成大规模的蛋白质网络，与NaOH-乙醇法获得的粗谷醇溶蛋白相比，粒径分布更窄。这项研究表明，RMs提取在从酿造工业副产品中提取醇溶蛋白方面具有潜在的应用，提供一种环保的白酒九灶回收方式。
