PDF(Pubmed)
Abstract：
Appropriate biodegradability to meet the demands of wound repair is critical for superficial wound repair membrane applications. Tyrosinase-catalyzed crosslinking SF (c-SF) membranes were constructed and regulated the degradation behavior in this study. The crosslinking degree of the c-SF membranes could be adjusted by reaction ratios of tyrosinase against SF (TYR/SF). Upon reaching a TYR/SF ratio of 20/6000, the degree of crosslinking increased to 88.17 ± 0.20%, without obvious changes in the crystal structure. The degradation behavior was regulated by the TYR/SF ratio and the degradation environment. All c-SF membranes remained stable after immersion without collagenase but showed an adjustable degradation behavior in the presence of collagenase. As the TYR/SF ratio increased, the residual weights increased from 23.31 ± 1.35% to 60.12 ± 0.82% after 7 days of degradation, occurring with low increased amounts of β-sheet structure and free amino acids. This work provides a new c-SF membrane with controllable rapid degradability and favorable cytocompatibility, which can help to meet requirements for biodegradable superficial wound repair membranes.
摘要：
适当的生物降解性以满足伤口修复的需求对于表面伤口修复膜应用是关键的。构建了酪氨酸酶催化的交联SF（c-SF）膜，并调节了其降解行为。c-SF膜的交联度可以通过酪氨酸酶与SF的反应比(TYR/SF)来调节。当TYR/SF比率达到20/6000时，交联度增加到88.17±0.20%，晶体结构无明显变化。降解行为受TYR/SF比值和降解环境的调节。所有c-SF膜在没有胶原酶的浸渍后保持稳定，但在胶原酶存在下显示出可调节的降解行为。随着TYR/SF比率的增加，降解7天后，残留量从23.31±1.35%增加到60.12±0.82%，发生在少量增加的β-折叠结构和游离氨基酸中。这项工作提供了一种新的c-SF膜，具有可控的快速降解性和良好的细胞相容性，这可以帮助满足可生物降解的浅层伤口修复膜的要求。
