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Abstract：
Background: Microbial dysbiosis may contribute to alpha-synuclein (α-Syn) homeostasis disruption, yet the burden of inflammatory periodontal infection and its treatment have never been studied in this regard. We aimed to compare the cytokine and α-Syn levels in the saliva and blood of patients with periodontitis who underwent non-surgical periodontal therapy (NSPT) and those of their healthy counterparts. Methods: Periodontal examination and saliva and blood sample collection were carried out in incoming patients at a university clinic. The periodontitis group (PG) received NSPT. The sample collection and periodontal observation were repeated 30 days after. IL-6, IL1-β and total α-Syn were quantified using immunoassay methods. The periodontal inflamed surface area (PISA) was calculated as a proxy for periodontal inflammation. Results: Eleven participants formed the PG, and there were fifteen healthy controls (HC). At baseline, no correlation between salivary and plasma α-Syn was found. The salivary α-Syn levels revealed a tendency to decrease 30 days after, particularly in the PD cases. The variation in PISA and α-Syn showed significant correlation. Salivary α-Syn correlated negatively with salivary IL-6 levels at both timepoints in the total sample (rho = -0.394 and rho = -0.451) and in the HC (rho = -0.632 and rho = -0.561). Variations in plasma IL-6 and α-Syn were negatively correlated (rho = -0.518) in the healthy participants. Baseline plasma IL1-β negatively correlated with plasmatic α-Syn at 30 days in the HC (rho = -0.581). Conclusions: Salivary and plasma α-Syn bioavailability operate independently, and periodontal diagnosis was not a confounding factor. Salivary α-Syn levels were significantly affected by NSPT, contrary to plasma levels. These results should be confirmed in future larger and prospective studies.
摘要：
背景：微生物菌群失调可能导致α-突触核蛋白（α-Syn）稳态破坏，然而，在这方面，尚未研究炎症性牙周感染的负担及其治疗。我们旨在比较接受非手术牙周治疗（NSPT）的牙周炎患者及其健康者的唾液和血液中的细胞因子和α-Syn水平。方法：在大学诊所对传入的患者进行牙周检查和唾液和血液样本收集。牙周炎组(PG)接收NSPT。30天后重复样品收集和牙周观察。使用免疫测定方法定量IL-6、IL1-β和总α-Syn。计算牙周发炎表面积（PISA）作为牙周炎症的代表。结果：11名参与者组成了PG，有15个健康对照（HC）。在基线,唾液和血浆α-Syn之间没有发现相关性。唾液α-Syn水平显示出30天后下降的趋势，特别是在PD病例中。PISA与α-Syn的变异呈显著相关。在总样品(rho=-0.394和rho=-0.451)和HC(rho=-0.632和rho=-0.561)的两个时间点,唾液α-Syn与唾液IL-6水平呈负相关。在健康参与者中，血浆IL-6和α-Syn的变化呈负相关（rho=-0.518）。在HC中30天，基线血浆IL1-β与血浆α-Syn呈负相关（rho=-0.581）。结论:唾液和血浆α-Syn生物利用度独立运行,牙周诊断不是混杂因素。唾液α-Syn水平受NSPT显著影响，与血浆水平相反。这些结果应该在未来更大的前瞻性研究中得到证实。
