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Abstract：
Background and Objective: Colorectal cancer (CRC) is among the most common types of cancer. Although the disease is treatable in its early stages, five-year survival falls below 20% in the later stages. CEA and CA19-9 are tumor markers used in the diagnosis and follow-up of the disease in clinical practice; however, their diagnostic effectiveness is insufficient. Therefore, the identification of biomarkers that can be easily studied from serum and can diagnose CRC and determine its severity is highly important. In this context, dickkopf1 (DKK1) and cytoskeleton-associated protein 4 (CKAP4) are both promising biomarkers. Materials and Methods: Serum DKK1 and CKAP4 levels were measured in 55 patients with CRC and 40 healthy controls. The patients with CRC were divided into groups based on pathological stages and histological differentiation. The serum levels of both proteins in patients with CRC were measured preoperatively and 10 and 30 days postoperatively. Results: Serum DKK1 and CKAP4 were significantly higher in the CRC group than in the healthy controls (p < 0.05). Serum levels of both proteins rose in line with the disease stage and grade but decreased following surgical resection. A positive correlation was observed between tumor diameter and protein blood levels. The diagnostic efficacy of DKK1 and CKAP4 in CRC (approximately 95%) was higher than that of markers such as CEA and CA19-9. Conclusions: The DKK1 and CKAP4 serum values of patients with CRC are promising biomarkers. They can potentially be used in CRC management, namely, in the diagnosis and treatment of tumor response access and in tumor aggressiveness prediction.
摘要：
背景与目的：结直肠癌（CRC）是最常见的癌症类型之一。虽然这种疾病在早期是可以治疗的，5年生存率在后期下降到20%以下.CEA和CA19-9是临床上用于疾病诊断和随访的肿瘤标志物；然而，其诊断效果不足。因此,鉴定可以从血清中轻松研究并可以诊断CRC并确定其严重程度的生物标志物非常重要。在这种情况下，dickkopf1（DKK1）和细胞骨架相关蛋白4（CKAP4）都是有前途的生物标志物。材料与方法：检测55例CRC患者和40例健康对照者的血清DKK1和CKAP4水平。根据病理分期和组织学分化将CRC患者分组。术前和术后10天和30天测量CRC患者两种蛋白质的血清水平。结果：CRC组血清DKK1和CKAP4明显高于健康对照组（p<0.05）。两种蛋白质的血清水平与疾病分期和分级一致，但在手术切除后下降。肿瘤直径与血液蛋白水平呈正相关。DKK1和CKAP4在CRC中的诊断效力(约95%)高于CEA和CA19-9等标志物。结论：CRC患者的DKK1和CKAP4血清值是有希望的生物标志物。它们可能用于CRC管理，即,在肿瘤反应通路的诊断和治疗以及肿瘤侵袭性预测中。
