PDF(Pubmed)
Abstract：
Blastocystis spp. and Giardia duodenalis are two prevalent zoonotic intestinal parasites that can cause severe diarrhea and intestinal diseases in humans and many animals. Black goat (Capra hircus) farming is increasingly important in China due to the remarkable adaptability, high reproductive performance, rapid growth rate, and significant economic value of black goats. A number of studies have indicated that black goats are the potential reservoir of multiple zoonotic protozoans in China; however, the prevalence and zoonotic status of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province is still unknown. Thus, a total of 1200 fecal samples of black goats were collected from several representative regions at different altitudes in Shanxi Province and were examined for the presence and genotypes of G. duodenallis and Blastocystis spp. by amplifying the beta-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) loci of G. duodenalis and SSU rRNA of Blastocystis spp. using PCR and sequence analysis methods, respectively. The overall prevalence of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province were 7.5% and 3.5%, respectively. Two assemblages (B and E) of G. duodenalis and four subtypes (ST5, ST10, ST14, and ST30) of Blastocystis spp. were identified, with assemblage E and ST10 as the prevalent genotype and subtype in black goats, respectively. One novel multilocus genotype (MLG) was identified in MLG-E and was designated as MLG-E12. For both G. duodenalis and Blastocystis spp., the prevalence was significantly related to the region and age groups (p < 0.05). This is the first report on the prevalence of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province. These results not only provide baseline data for the prevention and control of both parasites in black goats in Shanxi Province, but also enhance our understanding of the genetic composition and zoonotic potential of these two parasites.
摘要：
囊胚。和十二指肠贾第鞭毛虫是两种常见的人畜共患肠道寄生虫，可引起人类和许多动物的严重腹泻和肠道疾病。黑山羊(Caprahircus)养殖由于其显著的适应性,在中国越来越重要,高繁殖性能，快速增长，和重要的经济价值的黑山羊。大量研究表明,黑山羊是我国多种人畜共患原生动物的潜在储集地,十二指肠芽孢杆菌和囊胚菌的患病率和人畜共患状况。在山西省的黑山羊仍然是未知的。因此，从山西省不同海拔高度的几个代表性地区收集了1200只黑山羊的粪便样本，并检查了十二指肠球茎和囊胚的存在和基因型。通过放大beta-giardin（bg），谷氨酸脱氢酶（gdh），和十二指肠G的磷酸三糖异构酶（tpi）基因座和囊胚的SSUrRNA。使用PCR和序列分析方法，分别。十二指肠G和囊胚的总体患病率。在山西省黑山羊中分别为7.5%和3.5%，分别。十二指肠G的两个组合（B和E）和囊胚的四个亚型（ST5，ST10，ST14和ST30）。被确认,以E和ST10组合为黑山羊的流行基因型和亚型，分别。在MLG-E中鉴定了一种新的多位点基因型(MLG),并将其命名为MLG-E12。对于十二指肠芽孢杆菌和囊胚菌。,患病率与地区和年龄组显著相关(p<0.05).这是有关十二指肠G.和囊胚的患病率的第一份报告。在山西省的黑山羊。这些结果不仅为山西省黑山羊两种寄生虫的防控提供了基线数据,而且也增强了我们对这两种寄生虫的遗传组成和人畜共患潜力的理解。
