PDF(Pubmed)
Abstract：
3-nitropropionic acid (3NPA) has been proposed as an useful modifier to mitigate ruminal enteric methane emissions. However, few studies investigated the effects of 3NPA on ruminal fermentation characteristics of grazing ruminants in vitro. Rumen fluid from grazing yak and cattle were collected and incubated with additions of 0, 8, and 16 mM 3NPA. The total gas production, CH4 production, and dry matter digestibility significantly decreased with increasing 3NPA doses in both ruminant species (p < 0.05) and methane production decreased to almost 100% in cattle at 8 mM NPA but not yak, while H2 accumulation showed an opposite trend. The total fatty acid (TVFA) production, acetate concentration, and propionate concentration in cattle decreased as 3NPA doses increased at 12 and 24 h incubation. For yak, the H2 accumulation reached its apex at 8 mM NPA (p < 0.05). The TVFA in yak decreased significantly with increasing 3NPA doses at 12 and 72 h incubation. Moreover, the acetate concentration and propionate concentration in yak decreased as 3NPA doses increased at 12 and 24 h incubation. Overall, these findings demonstrated that 3NPA could be used as a strategy to mitigate methane emissions; although, it negatively affected the dry matter degradability in vitro.
摘要：
3-硝基丙酸（3NPA）已被提出作为一种有用的改性剂，以减轻瘤胃肠甲烷的排放。然而,3NPA对放牧反刍动物瘤胃发酵特性的影响研究较少。收集来自放牧牦牛和牛的瘤胃液并与添加0、8和16mM3NPA一起孵育。天然气总产量,CH4生产，和干物质消化率随着3NPA剂量的增加而显着降低两种反刍动物（p<0.05），并且甲烷产量在8mMNPA的牛中降低到几乎100％，而不是牦牛，而H2积累则表现出相反的趋势。总脂肪酸(TVFA)产量，醋酸盐浓度，在孵育12和24小时时，随着3NPA剂量的增加，牛的丙酸浓度降低。对牦牛来说,H2积累在8mMNPA时达到顶点(p<0.05)。在孵育12和72小时时，随着3NPA剂量的增加，牦牛的TVFA显着降低。此外，在12和24小时孵育时，随着3NPA剂量的增加，牦牛体内的乙酸盐浓度和丙酸盐浓度降低。总的来说,这些研究结果表明，3NPA可以用作减少甲烷排放的策略；尽管，它对体外干物质降解性产生负面影响。
