PDF(Pubmed)
Abstract：
Hemolysis is a common cause of errors in laboratory tests as it affects blood parameters and leads to a positive or negative bias. This study aims to examine the relationship between the level of hemolysis (expressed as cell-free hemoglobin concentration, g/L) and the variability of metabolic and endocrine parameters and to determine the threshold level of hemolysis that causes an analytically and clinically significant bias for the twenty most frequently examined blood parameters in cows. Paired blood samples of 10 mL each were obtained from 30 cows. One was subjected to mechanical trauma and plasma was extracted directly from the other. Hemolyzed and non-hemolyzed samples from the same animal were mixed to obtain final samples with cell-free hemoglobin concentrations of 0, 1, 2, 4, 6, 8, and 10 g/L. Metabolic and endocrine parameters were measured in the samples and their deviation and the linear equation between the level of hemolysis and the deviation were determined. The following threshold values of hemolysis were determined, which correspond to the acceptable analytical (lower value) and clinical (upper value) levels of parameter variability: BHB 0.96 and 4.81; NEFA 0.39 and 3.31; GLU 0.38 and 3.90; ALB 1.12 and 6.11; TPROT 1.40 and 6.80; UREA 6.62 and 20.1; TBIL 0.75 and 5.65; AST 0.11 and 2.18; GGT 1.71 and 8.90, LDH 0.01 and 0.11, ALP 0.97 and 2.95; TGC 1.56 and 15.5; CHOL 1.29 and 8.56; Ca 5.68 and 25.7; P 0.57 and 8.43; Mg 1.10 and 8.47; INS 1.15 and 3.89; T3 8.19 and 15.6; T4 8.97 and 18.5; and CORT 2.78 and 11.22 g/L cell-free hemoglobin. Three decision levels are available for each metabolic and endocrine parameter: if hemolysis is below the lower (analytical) threshold value, results can be reported without restriction; if hemolysis is between the lower and upper thresholds, the results can be issued with guidance in the form of corrective linear equations; and if hemolysis is above the upper (clinical) threshold, the results and sample must be discarded. This method contributes to an optimal approach to hemolysis interference with metabolic profile parameters in blood samples from cows.
摘要：
溶血是实验室测试中错误的常见原因，因为它会影响血液参数并导致正面或负面偏差。这项研究旨在检查溶血水平之间的关系（表示为无细胞血红蛋白浓度，g/L）以及代谢和内分泌参数的变异性，并确定溶血的阈值水平，该阈值水平会导致奶牛中20种最常检查的血液参数的分析和临床上的显着偏差。从30头母牛获得每个10mL的配对血液样品。一个遭受机械创伤，血浆直接从另一个中提取。将来自同一动物的溶血和非溶血样品混合，以获得无细胞血红蛋白浓度为0、1、2、4、6、8和10g/L的最终样品。测量了样品中的代谢和内分泌参数，并确定了它们的偏差以及溶血水平与偏差之间的线性方程。确定了以下溶血阈值，与参数变异性的可接受分析（较低值）和临床（较高值）水平相对应：BHB0.96和4.81；NEFA0.39和3.31；GLU0.38和3.90；ALB1.12和6.11；TPROT1.40和6.80；UREA6.62和20.1；TBIL0.75和5.65；AST0.11和2.18；GGT1.71和1.82.56mg和1.78每个代谢和内分泌参数有三个决定水平：如果溶血低于较低（分析）阈值，结果可以不受限制地报告；如果溶血在下限和上限阈值之间，结果可以以校正线性方程的形式提供指导；如果溶血高于上限（临床）阈值，结果和样本必须丢弃。该方法有助于对来自奶牛的血液样品中的代谢谱参数进行溶血干扰的最佳方法。
